Analysis of the heat stress transcription factors network of Arabidopsis thaliana

拟南芥热应激转录因子网络分析

• 批准号: 5352758
• 负责人: Professor Dr. Lutz Nover
• 金额: --
• 依托单位: Institut für Molekulare Biowissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2003-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5352758?language=en
• 关键词: Analysis; heat; stress; transcription; factors

The complexity of the plant stress response systems correlates with an unusual multiplicity of regulatory proteins. Analysis of the Arabidopsis genome revealed the existence of 21 ORFs encoding heat stress transcription factors (Hsfs). The project aims at the comparative analysis of these Hsfs with respect to their expression patterns, activator functions, intracellular localization and above all their interaction with other Hsfs (heterooligomerization) or other regulatory proteins (coregulators). A central aspect will be the characterization of the complex promoter architecture of the Arabidopsis hs genes and the role of hsf combinations for efficient activation of these genes (synergism between Hsfs). The results from the first three years period will serve as a basis for more advanced investigations of the Hsf network and its cross talks with the general stress signalling pathways of plants.

植物应激反应系统的复杂性与异常多样的调节蛋白相关。对拟南芥基因组的分析揭示了编码热应激转录因子 (Hsfs) 的 21 个 ORF 的存在。该项目旨在对这些 Hsfs 的表达模式、激活剂功能、细胞内定位以及最重要的是它们与其他 Hsfs（异源寡聚化）或其他调节蛋白（共调节蛋白）的相互作用进行比较分析。一个核心方面是拟南芥 hs 基因复杂启动子结构的表征以及 hsf 组合在有效激活这些基因中的作用（Hsf 之间的协同作用）。前三年的结果将作为对 Hsf 网络及其与植物一般应激信号通路的交叉对话进行更高级研究的基础。