AtGenExpress: Ein multinationales Projekt zur Analyse des Transcriptomes der Modellpflanze Arabidopsis thaliana

用ATH1全基因组芯片进行表达谱分析的Affymetrix技术允许可靠地检测大约24,000个拟南芥的蛋白质编码基因。它包括RNA加工和标记技术以及带有工作站和数据处理软件的基因阵列扫描仪。总而言之，这代表着全球范围内广泛使用的工具的独特组合。基于这项技术，AFGN小组提出了一个国际合作项目(AtGenExpress)，在DFG的资金支持下，生成一个涵盖约230个数据集的基因表达谱数据库，这些数据集来自500个RNA样本。这一部分包括拟南芥的发育、胁迫反应、光反应和病原物相互作用的样本。它将得到美国国家科学基金会2010年项目(美国)关于病原体反应的80个数据集、来自理研(日本)关于激素反应的170个数据集以及来自Tübingen和Golm的MPIs的约50个数据集的补充。来自1300多个微阵列的全部数据库，即3000多万个数据点，将作为国际基因表达总览(GEO)和ArraExpress数据库的一部分向公众发布。数据处理和出版将由国家科学基金会支持的拟南芥数据库(美国泰尔)管理。

The Affymetrix technology for expression profiling with the ATH1 full genome chip allows the reliable detection of about 24,000 protein-coding genes of Arabidopsis. It includes the RNA processing and labelling technology and the Gene Array Scanner with workstation and data processing software. Alltogether this represents a unique combination of tools broadly used worldwide. Based on this technology, the AFGN groups propose an international cooperation project (AtGenExpress) to generate a gene expression profiling database covering about 230 data sets derived from 500 RNA samples with financial support by the DFG. This part includes samples for Arabidopsis development, stress responses, light response and pathogen interaction. It will be complemented by 80 data sets from the NSF 2010 project (USA) on pathogen responses, by 170 data sets from RIKEN (Japan) on hormone responses and by about 50 data sets from the MPIs in Tübingen and Golm. The overall data base derived from more than 1300 microarrays, i.e. more than 30 million data points, will be released to the public as part of the international Gene Expression Omnibus (GEO) and ArraExpress data bases. Data processing and publication will be managed by the NSF-supported Arabidopsis database (TAIR, USA).