AtGenExpress: Ein multinationales Projekt zur Analyse des Transcriptomes der Modellpflanze Arabidopsis thaliana

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The Affymetrix technology for expression profiling with the ATH1 full genome chip allows the reliable detection of about 24,000 protein-coding genes of Arabidopsis. It includes the RNA processing and labelling technology and the Gene Array Scanner with workstation and data processing software. Alltogether this represents a unique combination of tools broadly used worldwide. Based on this technology, the AFGN groups propose an international cooperation project (AtGenExpress) to generate a gene expression profiling database covering about 230 data sets derived from 500 RNA samples with financial support by the DFG. This part includes samples for Arabidopsis development, stress responses, light response and pathogen interaction. It will be complemented by 80 data sets from the NSF 2010 project (USA) on pathogen responses, by 170 data sets from RIKEN (Japan) on hormone responses and by about 50 data sets from the MPIs in Tübingen and Golm. The overall data base derived from more than 1300 microarrays, i.e. more than 30 million data points, will be released to the public as part of the international Gene Expression Omnibus (GEO) and ArraExpress data bases. Data processing and publication will be managed by the NSF-supported Arabidopsis database (TAIR, USA).