Role of Bcl6 for generation of CD8+ dendritic cells

Bcl6 在 CD8 树突状细胞生成中的作用

• 批准号: 18590467
• 负责人: SAKAMOTO Akemi
• 金额: $ 2.57万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590467/
• 关键词: Immunology; allergy; target gene; gene regulation; development; 発王制御; BCL6; 樹状細胞; 転写因子; サイトカイン; BCL6欠損マウス; CD8陽性樹状細胞; CD4陽性樹状細胞; 抗原提示細胞

Bcl6-deficient mice display Th2-type inflammation and dendritic cells (DCs) are critical to induce Th1/2 cells. However, function of Bcl6-deficient DCs has never been discussed.We examined a role of Bcl6 in DC generation and function in this study. 1. The number of CD11c^<high> conventional DCs (cDCs) including CD8α^+ and CD4^+ DC subsets but not that of B220^+ mPDCA-1^+ plasmacytoid DCs (pDCs) was markedly reduced in the spleen of Bcl6-deficent mice. 2. Generation of cDCs but not that of pDCs was perturbed in BM chimerical mice. 3. Precursor (CD24^+CD11b^<low>) cells of CD8α^+ cDCs derived from Bcl6-deficient BM cells stimulated with Flt3L were apoptotic more than those from wild type BM cells. 4. High level of Bcl6 mRNA was detected in splenic CD4^+ and CD8α^+ cDCs. 5. LPS-activated Bcl6-deficient DCs produced IL-6 and induced Th2 cells more than LPS-activated wild type DCs did.Taken together Bcl6 in DC precursor cells is required for generation of CD4^+ and CD8α^+ cDCs and DCs might be one of the cause of Th2-type inflammation in Bcl6-deficient mice.

Bcl6 缺陷小鼠表现出 Th2 型炎症，而树突状细胞 (DC) 对于诱导 Th1/2 细胞至关重要。然而，Bcl6 缺陷型 DC 的功能从未被讨论过。我们在本研究中研究了 Bcl6 在 DC 生成和功能中的作用。 1. Bcl6缺陷小鼠脾脏中CD11c^<high>常规DC（cDC）的数量显着减少，包括CD8α^+和CD4^+ DC亚群，但B220^+ mPDCA-1^+浆细胞样DC（pDC）的数量不显着减少。 2. BM嵌合小鼠中cDC的产生受到干扰，但pDC的产生没有受到干扰。 3.用Flt3L刺激的源自Bcl6缺陷型BM细胞的CD8α^+cDC的前体(CD24^+CD11b^<low>)细胞比来自野生型BM细胞的细胞凋亡更多。 4. 在脾CD4^+和CD8α^+ cDC中检测到高水平的Bcl6 mRNA。 5. LPS激活的Bcl6缺陷型DC比LPS激活的野生型DC产生更多的IL-6并诱导Th2细胞。总而言之，DC前体细胞中的Bcl6是产生CD4^+和CD8α^+ cDC所必需的，并且DC可能是Bcl6缺陷小鼠中Th2型炎症的原因之一。

项目成果

Bc16 controls Granzyme B expression in effector CD8 T cells

Bc16 控制效应 CD8 T 细胞中颗粒酶 B 的表达

• 发表时间: 2006
• 期刊: Eur. J. Immunol. 36
• 作者: Yoshida;K.;Sakamoto;A.;Yamashita;K.;Arguni;E.;Horigome;S.;Arima;M.;Hatano;M.;Seki;N.;Ichikawa;T.;and Tokuhisa;T.
• 通讯作者: T.

Role for Bcl6 in memory T cell differentiation

Bcl6 在记忆 T 细胞分化中的作用

• 发表时间: 2007
• 作者: Akemi Sakamoto;et. al.;Akemi Sakamoto
• 通讯作者: Akemi Sakamoto

Role of Clast1 in differentiation of cerebellar granule cells.

Clast1 在小脑颗粒细胞分化中的作用。

• 发表时间: 2006
• 期刊: Brain Res. 1104
• 作者: Maeda;Y. et al.
• 通讯作者: Y. et al.

Bcl6 involved in the maintenance of non-polarized CD4 T cells

Bcl6 参与非极化 CD4 T 细胞的维持

• 发表时间: 2007
• 作者: 坂本 明美;他
• 通讯作者: 他

JunD/AP-1 and STAT3 are the major enhancer molecules for high Bcl6 expression in germinal center B cells

• DOI: 10.1093/intimm/dxl041
• 发表时间: 2006-07-01
• 期刊: INTERNATIONAL IMMUNOLOGY
• 影响因子: 4.4
• 作者: Arguni, Eggi;Arima, Masafumi;Tokuhisa, Takeshi
• 通讯作者: Tokuhisa, Takeshi