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Does angiogenesis require smooth muscular cells?

血管生成需要平滑肌细胞吗？

基本信息

批准号：
18591405
负责人：
SAKAKIBARA Yuzuru
金额：
$ 2.45万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591405/
关键词：
Regenerative Medicine Angiogenesis Ischemic limb Endothelial cell Smooth muscular cell Pericyte Arteriogenesis Mural Cell 再生医学放射光血管造影

项目摘要

1. Investigation in relation to Endothelial Cell/Pericyte Adhesion and angiogenesisAdhesive rate on capillary endothelial cell and pericyte was investigated in diabetic mice with hindlimb ischemia using Cilostazol, which is proved to be effective drug for peripheral vascular disease. Cilostazol-treated mice preserved both cell adhesion significantly compared with non-treated mice. At the same time, Cilostazol-treated mice showed higher rate of angiogenesis than non-treated mice significantly. This result implies that mural cells (pericytes and smooth muscle cells) are essential for angiogenesis. Moreover, their adhesion to endothelial cells is also important for angiogenesis.2. Investigation on visualization of arteriogenesis using synchrotron radiation micro-angiography (SRMA)Several types of endothelial progenitor cells (EPCs) were injected to ischemic hindlimb of nude mice. EPCs were classified according to Aldehyde Dehydrogenase activity. EPCs with low Aldehyde Dehydrogenase activity (Alde-low) showed higher vascular density (angiogenesis) than EPCs with high Aldehyde Dehydrogenase activity (Alde-high). At the same time, Alde-low showed higher number of arteriogenesis than Alde-high confirmed by SRMA. Since arteriogenesis was achieved by accumulation of smooth muscle cells in pre-existence arterioles, it is turned out that effective angiogenesis like application of Alde-low is essentially accompanied with smooth muscle cells.

1.内皮细胞/周细胞粘附与血管生成关系的研究用治疗周围血管疾病的有效药物西洛他唑（Cilostazol）对糖尿病小鼠后肢缺血模型的毛细血管内皮细胞和周细胞的粘附率进行了研究。与未处理的小鼠相比，西洛他唑处理的小鼠显著地保持了两种细胞粘附。同时，西洛他唑治疗的小鼠显示出比未治疗的小鼠显著更高的血管生成率。这一结果表明，壁细胞（周细胞和平滑肌细胞）是血管生成所必需的。此外，它们与内皮细胞的粘附对血管生成也很重要.同步辐射微血管造影（SRMA）显示血管新生的研究将几种内皮祖细胞（EPCs）注射到裸鼠缺血后肢。根据乙醛脱氢酶活性对EPCs进行分类。具有低醛脱氢酶活性（Alde-low）的EPCs比具有高醛脱氢酶活性（Alde-high）的EPCs显示更高的血管密度（血管生成）。同时，SRMA证实Alde-低比Alde-高显示出更高的动脉生成数量。由于动脉生成是通过平滑肌细胞在预先存在的小动脉中的积累来实现的，因此证明了有效的血管生成（如应用Alde-low）基本上伴随着平滑肌细胞。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effect of erythropoietin on vascular regeneration of myocardial infarction assessed bysynchrotron radiation coronary angiography.

同步辐射冠状动脉造影评估促红细胞生成素对心肌梗死血管再生的影响。

DOI：
发表时间：
2007
期刊：
Photon Factory Activity Report 2006 (Part B) 24
影响因子：
0
作者：
Matsushita S;Sato F;Sakakibara Y;et. al.
通讯作者：
et. al.

Effect of erythropoietin on vascular regeneration of myocardial infarction assessed by synchrotron radiation coronary angiography

同步辐射冠状动脉造影评估促红细胞生成素对心肌梗死血管再生的影响

DOI：
发表时间：
2007
期刊：
Photon Factory Activity Report 2006 24
影响因子：
0
作者：
Matsushita S;Sato F;Sakakibara Y;et. al.
通讯作者：
et. al.

Elucidation of Microvascular Response using Synchrotron Radiation Micro-Angiography.

使用同步辐射微血管造影阐明微血管反应。

DOI：
发表时间：
2007
期刊：
Photon Factory Activity Report 2006 (Part A) Highlight and Faculty Report 24
影响因子：
0
作者：
Matsushita S;Sato F;Sakakibara Y;et. al.
通讯作者：
et. al.

Effect of erythropoietin on vascular regeneration of myocardial infarction assessed by synchrotron radiation coronary angiography.

同步辐射冠状动脉造影评估促红细胞生成素对心肌梗死血管再生的影响。