A new culture system for studying in vitro human dental pulp repair
研究体外人牙髓修复的新培养系统
基本信息
- 批准号:18592093
- 负责人:
- 金额:$ 2.57万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2006
- 资助国家:日本
- 起止时间:2006 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In this study, we investigated the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month, Results shows that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation, neovascularization, and the presence of functional cuboidal cells close to the injured area. After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. Tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.The neurotransmitter peptide substance P (SP) is widely distributed in the central and peripheral nervous systems and also has been found in nonneural tissues. This one has been reported to play an immunoregulatory role in many physiological functions. SP stimulates T-cell proliferation in rat dentin-pulp complex and stimulates human monocytes to produce inflammatory cytokines, including interleukin (IL) -1, IL-6, IL-10, IL-12,tumor necrosis factor-α. Our results demonstrate that VR1 enhances LPS-induced expression of genes for inflammatory molecules in human dentin-pulp complex culture systems. In addition, our data show that LPS induces expression of VR1 in these cultures. This results suggest that LPS up-regulates the cellular response to VR1 in human dentin-pulp complex cultures.In summary, our findings show that VR1 plays important role in dentin-pulp complex inflammation and sensitivity.
在这项研究中,我们研究了拔牙后立即钻取的人牙厚片的行为,培养3天到1个月,结果表明,在体外,洞下受损的牙髓能够形成一些与组织愈合相关的典型方面,表现为细胞增殖,新生血管,以及损伤区域附近功能立方细胞的存在。培养30天后,可见细长的纺锤形细胞沿相关牙本质壁的边缘排列,而健康区域下功能良好的成牙本质细胞保存完好。神经递质P物质广泛分布于中枢和外周神经系统,在非神经组织中也广泛存在。据报道,它在许多生理功能中起着免疫调节作用。SP刺激大鼠牙本质-牙髓复合体T细胞增殖,刺激人单核细胞产生炎性细胞因子,包括IL-1、IL-6、IL-10、IL-12、肿瘤坏死因子-α。我们的结果表明,在人牙本质-牙髓复合培养系统中,VR1增强了内毒素诱导的炎症分子基因的表达。此外,我们的数据显示,在这些培养物中,内毒素可诱导VR1的表达。这一结果表明,在人牙本质-牙髓复合体培养中,内毒素上调了细胞对VR1的反应。综上所述,我们的研究结果表明,VR1在牙本质-牙髓复合体的炎症和敏感性中发挥着重要作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Roxithromycin inhibits tumor necrosis factor-α-induced matrix metalloproteinase-1 expression through regulating mitogen-activated protein kinase phosphorylation and Ets-1 expression
- DOI:10.1111/j.1600-0765.2006.00914.x
- 发表时间:2007-02-01
- 期刊:
- 影响因子:3.5
- 作者:Oyama, T.;Matsushita, K.;Torii, M.
- 通讯作者:Torii, M.
Runx2 is involved in the inhibition of MMP-13 expression by roxithromycin in human gingival cell cultures
Runx2 参与罗红霉素对人牙龈细胞培养物中 MMP-13 表达的抑制
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Tabuchi S;et. al.
- 通讯作者:et. al.
細胞におけるサブスタンスPのIL-6産生誘導に対するMAPキナーゼと転写因子の関与
MAP 激酶和转录因子参与细胞中 P 物质诱导 IL-6 的产生
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:徳田 雅行;他
- 通讯作者:他
The involvement of mitogen -activated protein kinase and transcriptional factors in substance P-induced IL-6 production in human dental pulp cell cultures
人牙髓细胞培养物中丝裂原激活蛋白激酶和转录因子参与 P 物质诱导的 IL-6 产生
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Tokuda;M.;Sakuta;T.;Oyama;T.;Tatsuyama;S.;Nagaoaka;S.;Torii;M
- 通讯作者:M
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NAGAOKA Shigetaka其他文献
NAGAOKA Shigetaka的其他文献
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{{ truncateString('NAGAOKA Shigetaka', 18)}}的其他基金
Development of analyzing system for studying human dental pulp repair
人体牙髓修复研究分析系统的开发
- 批准号:
12671856 - 财政年份:2000
- 资助金额:
$ 2.57万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Expression of enamel matrix protein in vitro
釉质基质蛋白的体外表达
- 批准号:
12557194 - 财政年份:2000
- 资助金额:
$ 2.57万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A new culture system for studying in vitro human dental pulp repair
研究体外人牙髓修复的新培养系统
- 批准号:
10671795 - 财政年份:1998
- 资助金额:
$ 2.57万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Possible pathogenic effect of Streptococcus mitis super antigen on oral epithelial cells
轻症链球菌超抗原对口腔上皮细胞可能的致病作用
- 批准号:
07457461 - 财政年份:1995
- 资助金额:
$ 2.57万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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