A new culture system for studying in vitro human dental pulp repair

在这项研究中，我们研究了拔牙后立即钻取的人类牙齿厚片的行为，并培养了3天至1个月，结果表明，在体外，腔下受损的牙髓能够发育出一些与组织愈合相关的典型方面，包括细胞增殖、新生血管形成以及损伤区域附近存在功能性立方体细胞。经过30天的培养，可以看到细长的纺锤形细胞沿着相关牙本质壁的边缘排列，而功能健全的成牙本质细胞在健康区域下方保存完好。组织恢复使我们相信，这种培养模型将是一个有用的系统测试因素调节牙髓修复。神经递质物质P （SP）广泛分布于中枢和周围神经系统，在非神经组织中也有发现。据报道，该基因在许多生理功能中发挥免疫调节作用。SP刺激大鼠牙本质-牙髓复合体t细胞增殖，刺激人单核细胞产生炎性细胞因子，包括白细胞介素(IL) -1、IL-6、IL-10、IL-12、肿瘤坏死因子-α。我们的研究结果表明，VR1增强了lps诱导的人牙本质-牙髓复合体培养系统中炎症分子基因的表达。此外，我们的数据显示LPS在这些培养中诱导VR1的表达。这些结果表明，LPS上调了人牙本质-牙髓复合体培养中VR1的细胞反应。综上所述，我们的研究结果表明VR1在牙本质-牙髓复合体炎症和敏感性中起重要作用。

In this study, we investigated the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month, Results shows that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation, neovascularization, and the presence of functional cuboidal cells close to the injured area. After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. Tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.The neurotransmitter peptide substance P (SP) is widely distributed in the central and peripheral nervous systems and also has been found in nonneural tissues. This one has been reported to play an immunoregulatory role in many physiological functions. SP stimulates T-cell proliferation in rat dentin-pulp complex and stimulates human monocytes to produce inflammatory cytokines, including interleukin (IL) -1, IL-6, IL-10, IL-12,tumor necrosis factor-α. Our results demonstrate that VR1 enhances LPS-induced expression of genes for inflammatory molecules in human dentin-pulp complex culture systems. In addition, our data show that LPS induces expression of VR1 in these cultures. This results suggest that LPS up-regulates the cellular response to VR1 in human dentin-pulp complex cultures.In summary, our findings show that VR1 plays important role in dentin-pulp complex inflammation and sensitivity.