A new culture system for studying in vitro human dental pulp repair

在这项研究中，我们研究了拔牙后立即钻取的人类牙齿厚片的行为，并培养3天至1个月。结果表明，牙髓损伤在体外能够发育出与组织愈合相关的一些典型方面，包括细胞增殖、新生血管形成和靠近损伤区域的功能性立方体细胞的存在。经过30天的培养，可以看到细长的纺锤形细胞沿着相关牙本质壁的边缘排列，而功能健全的成牙本质细胞在健康区域下方保存完好。组织恢复使我们相信，这种培养模型将是一个有用的系统测试因素调节牙髓修复。

In this study, we investigated the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month. Results show that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation, neovascularization, and the presence of functional cuboidal cells close to the injured area. After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. Tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.