A new culture system for studying in vitro human dental pulp repair
A new culture system for studying in vitro human dental pulp repair
批准号:
10671795
负责人:
NAGAOKA Shigetaka
金额:
$2.11万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 1999
中文摘要
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英文摘要
In this study, we investigated the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month. Results show that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation, neovascularization, and the presence of functional cuboidal cells close to the injured area. After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. Tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.
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Tanuma, J., Shisa, H., Hiai, H., Higashi, S., Yamada, Y., Kamoto, T., Hirayama, Y., Matsuuchi, H. & Kitano M.: "Quantitative trait loci affecting 4NQO-induced tongue carcinogenesis in the rat."Cancer Res.. 56. 1660-1664 (1998)
田沼 J.、志沙 H.、比合 H.、东 S.、山田 Y.、加本 T.、平山 Y.、松内 H.
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Miura, Y., Ozaki, H.S., Li, T-J., Uemura,M. & Kitano M.: "Experimental odontogenic cysts induced by in vitro 4-nitroquinoline 1-oxide (4NQO) treatment of F344 rat incisor tooth germs."J. Oral Pathol. Med.. 27. 53-58 (1998)
三浦 Y.、尾崎 H.S.、李 T-J.、上村 M.
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Kuniyoshi, H., Kitano, M., Uchio, Y., Enosa, E. T., Aloaina, L. F., Asaua, F. V. & Terashi, S.: "Seroepidermiological survey of anti-human T-cell leukemia virus, type 1 antibodies in Western Samoa."South Pacific Study. 18. 51-56 (1998)
Kuniyoshi, H.、Kitano, M.、Uchio, Y.、Enosa, E. T.、Aloaina, L. F.、Asaua, F. V.
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Miura, Y. et al.: "Experimental odontogenic cysts induced by ....."J. Oral. Pathol. med.. 27. 53-58 (1998)
Miura, Y. 等人:“实验性牙源性囊肿由……诱导”J.
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Matsushita, K., Tajima, T., Tomita, K., Abeyama, K., Maruyama, I., Takada, H. & Nagaoka, S.: "Inflammatory cytokine production and specific antibody responses against lipopolysaccharide from endodontopathic black-pigmented bacteria in patients with multil
松下,K.,田岛,T.,富田,K.,阿部山,K.,丸山,I.,高田,H.
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共 25 条
A new culture system for studying in vitro human dental pulp repair
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批准号:18592093
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.57万
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负责人:NAGAOKA Shigetaka
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Development of analyzing system for studying human dental pulp repair
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负责人:NAGAOKA Shigetaka
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