Development of analyzing system for studying human dental pulp repair

人体牙髓修复研究分析系统的开发

• 批准号: 12671856
• 负责人: NAGAOKA Shigetaka
• 金额: $ 2.05万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671856/
• 关键词: dentin-pulp complex; restorative material; pulp capping agent; 象牙質・歯髄複合体

In this study, we investigated the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month. Results show that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation, neovascularization, and the presence of functional cuboidal cells close to the injured area. After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. Tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.

在这项研究中，我们研究了厚片的行为，从人的牙齿钻孔后立即提取和培养3天至1个月。结果表明，腔下方的受损牙髓能够在体外发展与组织愈合相关的一些典型方面，这通过细胞增殖、新血管形成和靠近损伤区域的功能性立方细胞的存在来证明。培养30天后，可以看到细长的梭形细胞沿着相关牙本质壁的边缘排列，而健康区域下方的成牙本质细胞功能良好。组织恢复使我们相信，这样的文化模式将是一个有用的系统，用于测试调节牙髓修复的因素。

项目成果

Matsushita, K. et al.: "The role of vascular endothelial growth factor…"J. Dent. Res.. 79. 1596-1603 (2000)

Matsushita, K. 等人：“血管内皮生长因子的作用……”J. Dent. 79. 1596-1603 (2000)

Tokuda, M. et al.: "Regulation of interleukin-6 expression in…"J. Endodon.. 27. 273-277 (2001)

Tokuda, M. 等人：“白细胞介素 6 表达的调节......” J. Endodon.. 27. 273-277 (2001)

Oyama, T. et al.: "Effects of Roxithromycinon Tumor Neucresis…"J. Periodont.. 71. 1546-1553 (2000)

Oyama, T. 等人：“罗红霉素肿瘤坏死的影响……”J. periodont.. 71. 1546-1553 (2000)

Tokuda,M., Nagaoka,S. & Torii,M.: "Interleukin-10 inhibits expression of interleukin-6 and -8 mRNA in human dental pulp cell cultures via nuclear factor-κ B deactivation"J. Endodon. 28. 177-180 (2002)

Tokuda, M.、Nagaoka, S. 和 Torii, M.：“Interleukin-10 通过核因子 κ B 失活抑制人牙髓细胞培养物中 IL-6 和 -8 mRNA 的表达”J. Endodon。 -180 (2002)

Sakuta, T. et al.: "Enhanced production of vascular endothelial…"J. Med. Mierobiol.. 50. 233-237 (2001)

Sakuta, T. 等人：“增强血管内皮细胞的产生……”J. Mierobiol.. 50. 233-237 (2001)