The efficacy of biotin on the skin diseases related to dental Rats a

采用掌部脓疱性银屑病面积严重指数(PPPASI)对18例PPP患者进行皮疹严重程度评分，观察PPP患者是否存在骨性关节炎、牙周炎及生物素治疗效果。测定治疗前后患者外周血中CD4/8比值及血浆中肿瘤坏死因子-α、白介素6和生物素浓度。采用竞争性酶联分析法检测血清生物素浓度，发现骨性关节炎(39%)、牙周炎(56%)在这些患者中非常常见。然而，这些并发症与皮疹的严重程度无关。我们的4名患者检测到血浆IL-6浓度升高，尽管没有显著差异。CD_4/8和肿瘤坏死因子-α的变化。在这4例患者中，3例为重度牙周炎，1例为肾盂肾炎。每天服用9 mg或12 mg生物素的患者血清生物素浓度约为未服用生物素的患者的100倍。每日服用9 mg和12 mg的患者血清生物素浓度差异无统计学意义。这一结果表明，每天服用生物素9 mg就足以治疗PPP患者。生物素对14例(78%)患者非常有效。经生物素治疗后，患者的PPPASI明显降低(p&lt；0.01)。生物素对骨性关节炎也有效。经生物素治疗后，血清IL-6浓度显著降低(p&lt；0.01)。此外，我们还证实了牙科治疗对PPP患者牙疹和骨关节炎的协同作用。这些结果表明，IL-6可能是PPP患者严重牙周炎的有用标记物。我们还建议生物素(每天9毫克)配合牙周炎的牙科治疗对PPP是相当有效的。

We investigated 18 patients with pustular palmoplantaris (PPP) on the existence of osteoarthritis, periodontitis and the efficacy of biotin by scoring the severity of eruption with PPPASI (palmoplantar Pustular Psoriasis Area Severity Index). We also analyzed CD4/8 and plasma concentration of TNF-a , IL-6 and biotin before and after biotin treatment. Serum concentration of biotin was measured by competitive enzyme-linked assay.We found osteoarthritis (39%), periodontitis (56%) very frequently in these patients. However, these complications did not correlate with severity of eruptions. Elevations of plasma concentrations of IL-6 was detected in 4 of our patients, although there wasno significant. changes of CD4/8 and TNF-a . In these 4 cases three patients had severe periodontitis and one had pyelonephritisSerum concentration of biotin was about 100 times higher in the patients with administration of 9 or 12mg of biotin daily than those without biotin treatment. There was no significant difference of serum biotin concentration between patients with 9mg daily and those with 12mg daily. This result means 9mg of biotin per''day is enough for the treatment of the patients with PPP.Biotin was very effective for 14(78%)patients investigated. PPPASI of the patients was significantly lowered after biotin treatment (p<0.01). Biotin was also effective for the osteoarthritis. Serum IL-6 concentration was significantly decreased after biotin treatment (p<0.01). Furthermore, we confirmed synergic effect of dental treatment on eruptions and osteoarthritis of the patients with PPP.These results indicate IL-6 may be a useful marker for severe periodontitis in the patients with PPP. We also suggest biotin (9mg daily) together with dental treatment for periodontitis is quite effective for PPP.