The efficacy of biotin on the skin diseases related to dental Rats a

我们采用PPPASI（掌跖脓疱性银屑病面积严重性指数）评分法对18例掌跖脓疱性银屑病（PPP）患者的出疹严重程度进行调查，了解其骨关节炎、牙周炎的存在情况及生物素的疗效。我们还分析了生物素治疗前后CD4/8和血浆中TNF-a、IL-6和生物素的浓度。采用竞争酶联法测定血清生物素浓度。我们发现骨关节炎（39%）和牙周炎（56%）在这些患者中非常常见。然而，这些并发症与爆发的严重程度无关。在我们的4例患者中检测到血浆IL-6浓度升高，尽管没有显著性变化。CD4/8和TNF-a的变化。在这4例患者中，3例有严重牙周炎，1例有肾盂肾炎。每日给予生物素9或12mg的患者血清生物素浓度比未给予生物素治疗的患者高约100倍。9mg / d组与12mg / d组血清生物素浓度无显著差异。这一结果意味着每天9mg生物素足以治疗PPP患者。生物素对14例（78%）患者非常有效。生物素治疗后PPPASI明显降低（p<0.01）。生物素对骨关节炎也有效。生物素处理后血清IL-6浓度显著降低（p<0.01）。此外，我们还证实了牙齿治疗对骨性关节炎患者的牙疱和骨关节炎的协同作用。这些结果提示IL-6可能是PPP患者严重牙周炎的一个有用的标志物。我们也建议生物素（每天9mg）和牙周炎的牙科治疗对PPP非常有效。

We investigated 18 patients with pustular palmoplantaris (PPP) on the existence of osteoarthritis, periodontitis and the efficacy of biotin by scoring the severity of eruption with PPPASI (palmoplantar Pustular Psoriasis Area Severity Index). We also analyzed CD4/8 and plasma concentration of TNF-a , IL-6 and biotin before and after biotin treatment. Serum concentration of biotin was measured by competitive enzyme-linked assay.We found osteoarthritis (39%), periodontitis (56%) very frequently in these patients. However, these complications did not correlate with severity of eruptions. Elevations of plasma concentrations of IL-6 was detected in 4 of our patients, although there wasno significant. changes of CD4/8 and TNF-a . In these 4 cases three patients had severe periodontitis and one had pyelonephritisSerum concentration of biotin was about 100 times higher in the patients with administration of 9 or 12mg of biotin daily than those without biotin treatment. There was no significant difference of serum biotin concentration between patients with 9mg daily and those with 12mg daily. This result means 9mg of biotin per''day is enough for the treatment of the patients with PPP.Biotin was very effective for 14(78%)patients investigated. PPPASI of the patients was significantly lowered after biotin treatment (p<0.01). Biotin was also effective for the osteoarthritis. Serum IL-6 concentration was significantly decreased after biotin treatment (p<0.01). Furthermore, we confirmed synergic effect of dental treatment on eruptions and osteoarthritis of the patients with PPP.These results indicate IL-6 may be a useful marker for severe periodontitis in the patients with PPP. We also suggest biotin (9mg daily) together with dental treatment for periodontitis is quite effective for PPP.