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The usefulness of LTT and CAST in the diagnosis of metal allergy

LTT 和 CAST 在金属过敏诊断中的作用

基本信息

批准号：
12671965
负责人：
TAKAHASHI Shinichi
金额：
$ 1.66万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671965/
关键词：
metal allergy patch test LTT CAST

项目摘要

We performed LTT and CAST with commercially available metal series patch test solutions in 211 patients with metal allergy and 108 normal healthy volunteers and compared the result of each patch test. In LTT dilution series of patch test solution (50, 250, 6250, 31250 and 156520X) was propared as in the case of drug LTT and S.I.(%) of each dilution was measured after cultured 3 days or 6 days. In CAST background by metals was measured to establish optimal dilution series of patch test solution, and S.I. (%) of each solution was measured as in the case of food allergy test. In LTT of Pd, Au, Co, maximum S.I. For 3days culture was thought to be the optimal condition to assess metal allergy, although sensitivity is not very high and significance of longer culture was still to be determined. In LTT of Ni non-specific lymphocyte proliferation was observed at the concentration of maximum S.I. The maximum S.I. Of 200μg/ml 8ug/ml NiSO, for 3days culture was the most optimal value to represent Ni allergy. We need more positive patch test patients to determine the usefulness of 6 days culture. In LTT of Cr and Hg S.I. Value did not correlate to the results of patch test. LTT seems not to be useful to diagnose Cr or Hg allergy. In CAST non-specific positive reactions were not observed excopt in the lower concentration of Pd solution. However, not so many patients with metal allergy showed positive reactions. In Au S.I. Of the highest concentration was positive in several patients. In other metals sensitivity was very low. We need to investigate more patients to confirm these results. IN conclusion LTT of Ni, Pd, Au, Co can be used as a diagnostic method to assess metal allergy, although sensitivity is relatively low.

我们对211例金属过敏患者和108例正常健康志愿者进行了LTT和CAST，并比较了每种斑贴试验的结果。在LTT中，与LTT和S.I.药物一样，提供了斑贴试验溶液（50、250、6250、31250和156520 X）的系列稀释液。培养3天或6天后测量各稀释液的百分比（%）。在CAST背景中测定金属，以建立斑贴试验溶液的最佳稀释系列，并对S.I.与食物过敏试验的情况相同，测量每种溶液的浓度（%）。在Pd、Au、Co的LTT中，最大S.I. 3天培养被认为是评估金属过敏的最佳条件，尽管敏感性不是很高，并且更长时间培养的意义仍有待确定。在Ni的LTT中，在最大S.I.浓度下观察到非特异性淋巴细胞增殖。最大S.I.在200μg/ml 8 μ g/mlNiSO中，培养3d是代表镍过敏的最佳值。我们需要更多阳性斑贴试验患者来确定6天培养的有用性。在铬和汞S.I.值与斑贴试验结果无相关性。LTT对诊断Cr或Hg过敏似乎没有帮助。在CAST中，除较低浓度的Pd溶液外，未观察到非特异性阳性反应。然而，没有那么多的金属过敏患者表现出阳性反应。在Au S.I.最高浓度在几个病人中呈阳性。在其他金属中，灵敏度很低。我们需要调查更多的患者来证实这些结果。结论：镍、钯、Au、钴的LTT可作为金属过敏的诊断方法，但敏感性较低。