EVALUATION OF GRANDE OF MALLIGNANCY ON THE BASIS OF CELL CYCLE ADHESION MOLECULESIN ORAL SQUAMOUS CELL CARCUNOMA

抑癌基因p53的失调在多种人类恶性肿瘤的发生发展中起重要作用。一些研究者认为p53基因蛋白的累积表达可作为口腔鳞状细胞癌（OSCC）的预后指标，但也有人报道了相互矛盾的结果。本研究旨在探讨140例口腔鳞癌中p53基因蛋白的表达与临床病理特征的关系。从OSCC患者的肿瘤活检组织中获得的p53基因产物的免疫组化检测进行了检查，特别是与临床病理结果和总生存率相关的表达模式。p53在细胞核内的分布可分为边缘型、分散型和混合型。p53表达（阴性和阳性）的简单评估定义为10%的值，并不能提供有用的信息，以评估口腔癌。 ...更多信息 rsed型p53蛋白表达与淋巴结转移、肿瘤细胞分化程度、总生存期及突变密切相关。提示p53蛋白表达可能是口腔鳞癌恶性程度的生物学标志之一，而p53蛋白阳性则不是。抑癌基因的遗传学和表观遗传学改变在口腔鳞癌的发生中起重要作用。Ras信号通常在OSCC中被激活，尽管在OSCC患者中很少检测到RAS突变。在本研究中，我们检测RAS相关家族（RASSF）基因在口腔鳞癌中的表达和甲基化状态。RASSF 1（7/17：41.2%）、RASSF 2（12/17：70.6%）、RASSF 5（4/17：23.5%）在一组OSCC细胞系中以及46例OSCC患者标本中频繁检出（RASSF 1，6/46，13.0%; RASSF 2，12/46，26.1%; RASSF 5，5/46，10.9%）。我们的研究结果表明，RASSF的表观遗传失活在OSCC的发生中起着关键作用，RASSF可能是OSCC诊断和治疗的重要分子靶点。少

Dysregulation of tumor suppressor gene p53 has been shown to play a significant role in development of a wide variety of human malignancies. Some investigators have suggested that accumulated expression of p53 gene protein is useful as a prognostic indicator in oral squamous cell carcinoma (OSCC), although others have reported conflicting result. The aim of this study is to determine the correlation between the expression of p53 gene protein and the clinicopathological features of 140 OSCC. Tumor biopsies obtained from patients with OSCC were examined for the immunohistochemical detection of p53 gene product with special reference to expression pattern correlated with clinicopathological findings and overall survival. Nuclear accumulation of p53 was visualized as marginal pattern, dispersed pattern, or mixed pattern. Simple evaluation of p53 expression (negative and positive) defined with value of 10% did not provide useful information as to the assessment of oral.cancer However, dispe … More rsed type p53 protein expression significantly correlated with nodal metastasis, differentiation of tumor cell, overall survival and mutation. These finding suggest that p53 expression may, but not p53 positivity, be one of biological makers for the degree of malignancy in OSCC.Genetic and epigenetic alterations in tumor-suppressor genes play important roles in human neoplasia. Ras signaling is often activated in OSCC, although RAS mutations are rarely detected in OSCC patients. In present study, we examined the expression and and methylation statuses of RAS association family (RASSF) genes in OSCC. We frequently detected methlaion of RASSF1 (7/17:41.2%) RASSF2 (12/17:70.6%), RASSF5 (4/17:23.5%) in a panel of OSCC cell lines, as well as in specimens collected from 46 OSCC patient (RASSF1, 6/46, 13.0%; RAsSF2,12/46, 26.1%; RASSF5,5/46,10.9%). Our findings indicate that epigenetic inactivation of RASSF plays a key role in OSCC tumorigenesis, and that RASSF may be an important molecular target for the diagnosis and treatment of OSCC. Less