Bone regeneration for secondary bone graft and orthodontic evaluation

本研究的目的是：(1)如何使组织工程化骨用于CLP患者的二次植骨；(2)从正畸角度建立组织工程骨应用评价系统(S)。首先，通过RT-PCR检测胚胎干细胞(ES)在间充质细胞中的表达水平。其中未检测到Nanog基因。接下来，利用逆转录病毒载体将Nanog基因导入间充质细胞，以促进其向成骨细胞分化。结果表明，强制表达Nanog可促进间充质细胞向成骨方向分化。同时，强制表达Nanog可刺激传递BMP信号的Smad1/5/8，抑制在ES细胞自我更新中起重要作用的STAT3。根据这些结果，我们得出结论，阐明Nanog在成骨分化中的作用可能有助于了解骨代谢和骨再生医学。(157字)

The purposes of this study were ; (1) how to make tissue-engineered bone to be used in secondary bone graft for CLP patients and ; (2) to establish the system(s) to evaluate such application from an orthodontic viewpoint.First, we checked the expression levels of Nanog gene, which is essential to self-renewal of embryonic stem(ES) cells, among mesenchymal cells by performing RTPCR. There was not detectable level of Nanog gene among them. Next Nanog gene was introduced to mesenchymal cells with retrovirus vector aiming at improving osteogenic differentiation. As a result, forced Nanog expression could promote osteogenic differentiation of mesenchymal cells. At the same time, forced expression of Nanog caused stimulation of Smad1/5/8 which transmits BMP signal, and inhibition of STAT3 which plays an important role in self-renewal of ES cells. From these results, we concluded that elucidation of the role of Nanog in osteogenic differentiation may be useful for understanding bone metabolism and bone regenerative medicine. (157 words)