Bone regeneration for secondary bone graft and orthodontic evaluation

二次骨移植的骨再生和正畸评估

• 批准号: 18592231
• 负责人: SUSAMI Takafumi
• 金额: $ 2.53万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592231/
• 关键词: Bone Metabolism; Bone Regenerative Medicine; Dentistry; 骨分化; 顎裂部骨欠損; 歯科矯正学

The purposes of this study were ; (1) how to make tissue-engineered bone to be used in secondary bone graft for CLP patients and ; (2) to establish the system(s) to evaluate such application from an orthodontic viewpoint.First, we checked the expression levels of Nanog gene, which is essential to self-renewal of embryonic stem(ES) cells, among mesenchymal cells by performing RTPCR. There was not detectable level of Nanog gene among them. Next Nanog gene was introduced to mesenchymal cells with retrovirus vector aiming at improving osteogenic differentiation. As a result, forced Nanog expression could promote osteogenic differentiation of mesenchymal cells. At the same time, forced expression of Nanog caused stimulation of Smad1/5/8 which transmits BMP signal, and inhibition of STAT3 which plays an important role in self-renewal of ES cells. From these results, we concluded that elucidation of the role of Nanog in osteogenic differentiation may be useful for understanding bone metabolism and bone regenerative medicine. (157 words)

本研究的目的是；(1)如何制作组织工程骨用于CLP患者的二次骨移植；(2)建立系统，从正畸的角度评估这类应用。首先，我们通过RTPCR检测了Nanog基因在间充质细胞中的表达水平，该基因是胚胎干细胞自我更新所必需的。其中Nanog基因未检测到。利用逆转录病毒载体将Nanog基因导入间充质细胞，促进成骨分化。因此，强迫Nanog表达可以促进间充质细胞的成骨分化。同时，强迫表达Nanog会刺激传递BMP信号的Smad1/5/8，抑制在ES细胞自我更新中起重要作用的STAT3。从这些结果，我们得出结论，阐明Nanog在成骨分化中的作用可能有助于理解骨代谢和骨再生医学。(157字)

项目成果

Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes

• DOI: 10.1074/jbc.m608383200
• 发表时间: 2007-07-13
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Liu, Guangyao;Kawaguchi, Hiroshi;Hoshi, Kazuto
• 通讯作者: Hoshi, Kazuto

Comparison of unilateral cleft lip and palate treatments between several institutions

多家机构单侧唇腭裂治疗效果比较

• 发表时间: 2006
• 作者: Asahito T.;Ono K.;Morita S.;Mouri T.;Takagi R.,Terao E.;Kochi S.;Susami T.;Kurabayashi H.;Iwata T.;Tachimura S.;Negoro T.;Otsuka S.;Saito I
• 通讯作者: Saito I

Effects of TGF-β over chondrocytes used for Cartilage Tissue Engineering

TGF-β 对用于软骨组织工程的软骨细胞的影响

• 发表时间: 2006
• 作者: Ogasawara T.;Takahashi T.;Asawa Y.;Yamaoka H.;Tanaka Y.;Nishizawa s.;Liu G.;Yoda T.;Susami T.;Takato T.;Hoshi K.
• 通讯作者: Hoshi K.

Cytological roles of the FGF family in the chondrocyte redifferentiation

FGF家族在软骨细胞再分化中的细胞学作用

• 发表时间: 2007
• 作者: Nishizawa S.;Yamaoka H.;Ogasawara T.;Asawa Y.;Yamaoka K.;Takato T.;Hoshi K.
• 通讯作者: Hoshi K.

Cyclooxygenase-2 activity is essential for the osseointegration of dental implants

• DOI: 10.1016/j.ijom.2006.11.011
• 发表时间: 2007-05-01
• 期刊: INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
• 影响因子: 2.4
• 作者: Chikazu, D.;Tomizuka, K.;Takato, T.
• 通讯作者: Takato, T.