Regulation of keratinocyte differentiation and innate immunity by innate immune regulator Ubc13

先天免疫调节剂 Ubc13 对角质形成细胞分化和先天免疫的调节

• 批准号: 19390297
• 负责人: SAYAMA Koji
• 金额: $ 11.81万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390297/
• 关键词: 皮膚感染症; 自然免疫; 角化細胞; 細胞内シグナル; Ubc13; NF-kB; ノックアウトマウス; アポトーシス; 分化; MAP kinase; NF-кB; ユビキチン; TAK1; cre-recombinase; 皮膚

The E2 polyubiquitin-conjugating enzyme Ubc13 is a mediator of innate immune reactions. We generated keratinocyte-specific Ubc13-deficient mice (Ubc13^<flox/flox>K5-Cre). At birth, the skin of the Ubc13^<flox/flox>K5-Cre mice was abnormally shiny and smooth ; in addition, the mice did not grow and died by postnatal day P2. Histological analysis showed atrophy of the epidermis with keratinocyte apoptosis. Immunohistochemical analyses revealed reduced proliferation, abnormal differentiation, and apoptosis of keratinocytes in the Ubc13^<flox/flox>K5-Cre mouse epidermis. In culture, Ubc13^<flox/flox>K5-Cre keratinocyte growth was impaired and spontaneous cell death occurred. Therefore, Ubc13 is essential for keratinocyte growth, differentiation, and survival.

E2多聚泛素结合酶Ubc 13是先天免疫反应的介质。我们产生了角质形成细胞特异性Ubc 13缺陷小鼠（Ubc 13 ^<flox/flox>K5-Cre）。在出生时，Ubc 13 ^<flox/flox>K5-Cre小鼠的皮肤异常有光泽和光滑;此外，小鼠不生长并在出生后第P2天死亡。组织学分析显示表皮萎缩伴角质形成细胞凋亡。免疫组织化学分析显示Ubc 13 ^<flox/flox>K5-Cre小鼠表皮中角质形成细胞的增殖减少、分化异常和凋亡。在培养物中，Ubc 13 ^<flox/flox>K5-Cre角质形成细胞生长受损，并发生自发性细胞死亡。因此，Ubc 13对角质形成细胞的生长、分化和存活至关重要。

项目成果

The innate immunity mediator Ubc13-TAK1 is a master regulator of epidermal structure and apoptosis

先天免疫介质 Ubc13-TAK1 是表皮结构和细胞凋亡的主要调节因子

• 发表时间: 2008
• 作者: Sayama;K.
• 通讯作者: K.

Living skin equivalents constructed using human amnion as a matrix

使用人类羊膜作为基质构建的活体皮肤等效物

• 发表时间: 2009
• 期刊: J Dermatol Sci 56
• 作者: Yang L;et al.
• 通讯作者: et al.

Ablation of TAK1 in mouse keratinocytes disturbed hair follicle development

小鼠角质形成细胞中 TAK1 的消融扰乱了毛囊的发育

• 发表时间: 2007
• 作者: Sayama;K. Kajiya;K. Shirakata;Y. Hanakawa;Y. Hirakawa;S. Dai;X. Nagai;H. Akira;S. Kishimoto;J and Hashimoto;K
• 通讯作者: K

TAK1 regulates hair follicle development in EDA receptor signaling 37th Annual European Society for Dermatological Research(ESDR)Meeting

TAK1 通过 EDA 受体信号调节毛囊发育第 37 届欧洲皮肤病学研究学会 (ESDR) 年度会议

• 发表时间: 2007
• 作者: Sayama;K. Kajiya;K. Shirakata;Y. Hanakawa;Y. Hirakawa;S. Akira;S. Kishimoto;J. Hashimoto;K.
• 通讯作者: K.

CXCL 16 is a novel mediator of the innate immunity of epidermal keratinocytes Int Immunol

CXCL 16 是表皮角质形成细胞先天免疫的新型介质

• 发表时间: 2007
• 期刊: 19
• 作者: Tohyma;M. Sayama;K. Komatsuzawa;H. Hanakawa;Y. Shirakata;Y. Dai;X. Yang;L. Tokumaru;S. Nagai;H. Hirakawa;S. Sugai;M.;Hashimoto;K.
• 通讯作者: K.