Role of Atg5-dependent autophagy in innate immunity of epidermal keratinocytes

Atg5依赖性自噬在表皮角质形成细胞先天免疫中的作用

• 批准号: 22390218
• 负责人: SAYAMA Koji
• 金额: $ 12.06万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390218/
• 关键词: 皮膚感染症; 角化細; 自然免疫; オートファジー; Atg5; 角化細胞; ノックアウトマウス; 分化; LC; K5-Cre

Autophagy is a mechanism to degrade selective cellular components. Since autophagy is a mechanism degrading the intracellular components during starvation, autophagy may play an essential role in clearance of intracellular pathogens. The purpose of this study is to clarify the role of keratinocyte autophagy in innate immunity. Differentiation and starvation induced autophagy in cultured keratinocytes. Stimulation of Toll-like receptor ligands also induced autophagy, indicating that autophagy play an important role in epidermal innate immunity. The expression of LC3 was not altered in Atg5 KO mouse epidermis.

自噬是降解选择性细胞成分的机制。由于自噬是在饥饿期间降解细胞内组分的机制，因此自噬可能在细胞内病原体的清除中起重要作用。本研究的目的是阐明角质形成细胞自噬在天然免疫中的作用。分化和饥饿诱导角质形成细胞自噬。Toll样受体配体的刺激也诱导自噬，表明自噬在表皮天然免疫中起重要作用。在Atg5 KO小鼠表皮中，LC 3的表达没有改变。

项目成果

E2 Polyubiquitin-conjugating Enzyme Ubc13 in Keratinocytes Is Essential for Epidermal Integrity

• DOI: 10.1074/jbc.m110.106484
• 发表时间: 2010-09-24
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Sayama, Koji;Yamamoto, Masahiro;Hashimoto, Koji
• 通讯作者: Hashimoto, Koji

Mite allergen activates inflammasome in epidermal keratinocytes resulting in the production of IL-1beta and IL-18

螨过敏原激活表皮角质形成细胞中的炎症小体，导致产生 IL-1β 和 IL-18

• 发表时间: 2011
• 作者: Dai;X
• 通讯作者: X

Inflammatory mediator TAK1 regulates hair follicle morphogenesis and anagen induction shown by using keratinocyte-specific TAK1-deficient mice.

• DOI: 10.1371/journal.pone.0011275
• 发表时间: 2010-06-23
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Sayama K;Kajiya K;Sugawara K;Sato S;Hirakawa S;Shirakata Y;Hanakawa Y;Dai X;Ishimatsu-Tsuji Y;Metzger D;Chambon P;Akira S;Paus R;Kishimoto J;Hashimoto K
• 通讯作者: Hashimoto K

Autophagy is induced by differentiation in epidermal keratinocytes.

自噬是由表皮角质形成细胞的分化诱导的。

• 发表时间: 2012
• 作者: 1.Dai X;Okazaki H;Hanakawa Y;Shirakata Y;Murakami M;Tohyama M;and Sayama K.
• 通讯作者: and Sayama K.

Mite allergen is a danger signal for the skin

螨虫过敏原是皮肤的危险信号

• 发表时间: 2010
• 作者: Sayama K;Dai X;Shirakata Y;Tohyama M;Miyawaki S;Hirakawa S;Hashimoto K
• 通讯作者: Hashimoto K