Involvement of microflora-induced Th17 cells in psoriasis

微生物群诱导的 Th17 细胞参与银屑病

• 批准号: 22659207
• 负责人: SAYAMA Koji
• 金额: $ 2.07万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659207/
• 关键词: 無菌マウス; SPF; 自然免疫; Th17; 黄色ブドウ球菌; 表皮ブドウ球菌; TLR; 細菌感染; 尋常性乾癬; 角化細胞; インフラマソーム; 細菌; 表皮分化

Skin phenotypes of germ-free mice were compared with those of control SPF mice. The epidermis of germ-free mice was atrophic compared to SPF mice. Markers for keratinocyte differentiation such as keratins were histologically stained. Next, we studied whether pathogens can be a trigger for the development of psoriasis. S. aureus and S. epidermidis were heat-inactivated and added to the cultured keratinocytes. Cytokines and anti-microbial peptides were induced. Imiquimod, an activator of innate immunity, was used for the development of psoriatic skin lesions. Back skin of the mice was shaved and imiquimod was topically applied for the five consecutive days. One week after the application, erythema and scales appeared on the skin. Histological analyses confirmed the development of psoriasis.

将无菌小鼠的皮肤表型与对照SPF小鼠的皮肤表型进行比较。与SPF小鼠相比，无菌小鼠的表皮萎缩。角质形成细胞分化的标记物如角蛋白进行组织学染色。接下来，我们研究了病原体是否可以成为银屑病发展的触发因素。S.金黄色葡萄球菌和将表皮细胞热灭活并加入到培养的角质形成细胞中。诱导细胞因子和抗菌肽。咪喹莫特是一种先天免疫激活剂，用于银屑病皮肤病变的发展。将小鼠的背部皮肤剃毛，并连续五天局部施用咪喹莫特。施用后一周，皮肤上出现红斑和鳞屑。组织学分析证实了银屑病的发展。

项目成果

Inflammatory mediator TAK1 regulates hair follicle morphogenesis and anagen induction shown by using keratinocyte-specific TAK1-deficient mice.

• DOI: 10.1371/journal.pone.0011275
• 发表时间: 2010-06-23
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Sayama K;Kajiya K;Sugawara K;Sato S;Hirakawa S;Shirakata Y;Hanakawa Y;Dai X;Ishimatsu-Tsuji Y;Metzger D;Chambon P;Akira S;Paus R;Kishimoto J;Hashimoto K
• 通讯作者: Hashimoto K

E2 Polyubiquitin-conjugating Enzyme Ubc13 in Keratinocytes Is Essential for Epidermal Integrity

• DOI: 10.1074/jbc.m110.106484
• 发表时间: 2010-09-24
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Sayama, Koji;Yamamoto, Masahiro;Hashimoto, Koji
• 通讯作者: Hashimoto, Koji

Mite allergen is a danger signal for the skin via activation of the inflammasome in keratinocytes

螨过敏原是通过激活角质形成细胞中的炎性体向皮肤发出的危险信号

• 发表时间: 2011
• 期刊: J Allergy Clin Immunol
• 作者: Dai;X
• 通讯作者: X

PPARγ mediates innate immunity by regulating the 1α,25-dihydroxyvitamin D3 induced hBD-3 and cathelicidin in human keratinocytes

• DOI: 10.1016/j.jdermsci.2010.09.008
• 发表时间: 2010-12-01
• 期刊: JOURNAL OF DERMATOLOGICAL SCIENCE
• 影响因子: 4.6
• 作者: Dai, Xiuju;Sayama, Koji;Hashimoto, Koji
• 通讯作者: Hashimoto, Koji

Mite allergen activates inflammasome in epidermal keratinocytes resulting in the production of IL-1beta and IL-18

螨过敏原激活表皮角质形成细胞中的炎症小体，导致产生 IL-1β 和 IL-18

• 发表时间: 2011
• 作者: Dai;X
• 通讯作者: X