Alternative splicing of cancer-related genes in oral squamous cell carcinoma.

本研究的目的是通过研究口腔鳞癌（OSCC）患者样本中癌症相关基因剪接异构体的表达水平，寻找可能作为肿瘤靶向治疗分子的标志物。并分析了几种基因表达水平与临床病理因素的相关性。我们收集了107例OSCC患者的活检和手术样本，研究了MUC1、MUC4、VEGF和SULF2蛋白的表达水平。通过RT-PCR对55例OSCC患者口腔冲洗液中各癌相关基因的mRNA表达进行半定量分析。所得结果如下：1。sul2、MUC1、MUC4的表达水平与T分型高、转移到区域淋巴结、肿瘤分期晚期、预后不良相关。2。每位患者的癌症相关基因表达水平不同。3。构建MUC1、VEGF和VEGFR剪接变异体特异性引物，并通过培养细胞验证其敏感性和特异性。这些结果提示，sul2、MUC1和MUC4基因的表达水平可能是影响OSCC患者预后的新的有用因素。

The purpose of the present study is to find markers, which might be molecules of targeted cancer therapy for the patients with oral squamous cell carcinoma(OSCC),by means of investigating the expression level of splicing isoforms of cancer-related genes in samples from patients with OSCC. The correlation between the expression level of several genes and clinicopathological factors was also evaluated. We harvested samples of the biopsy and operation in 107 patients with OSCC,and investigated the expression level of proteins of MUC1, MUC4, VEGF, and SULF2. Also mRNA expression of each cancer-related genes in the oral rinse obtained from 55 patients with OSCC was semi-quantitated by RT-PCR.The results obtained are as follows :I. The expression level of SULF2, MUC1 and MUC4 was significantly correlated with higher T classification, metastasis to regional lymph node, advanced tumor stage and poor prognosis. II. The expression levels of cancer-related genes were varied in each patient. III. The specific primer sets for MUC1, VEGF and VEGFR splicing variants were conducted, and those sensitivity and specificity were confirmed by using cultured cell. These results suggest that the expression level of SULF2, MUC1 and MUC4 gene might be the new useful prognostic factors of OSCC patients.