Electron microscopic study on oral lesions of HIV-infection

HIV感染者口腔病变的电镜研究

• 批准号: 12671952
• 负责人: SUGIHARA Kazumasa
• 金额: $ 1.86万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671952/
• 关键词: HIV; Oral lesion; Oral Candidasis; HAART; Scanning electron microscopy; Transmission electron microscopy; ヒト免疫不全ウイルス; 電子顕微鏡; HIV関連歯周疾患

We investigated on oral lesions of 50 HIV-seropositive patients treated with highly active antiretroviral therapy for over 6 months at the Department of Infectious Disease in Charite Hospital, Humboldt University in Berlin. The patients consisted of 42 men and 8 women with a mean age of 41.5 years (24-61 years). 29 patients were homosexuals, 6 patients were heterosexuals, 7 patients were drug abusers, and 8 patients were others. Distribution of CDC-classification was 1 in A1, 8 in A2, 1 in A3, 1 in B1, 6 in B2, 13 in B3 and 20 in C3. Oral candidiasis was observed in 27 patients, oral candidiasis with other oral lesions was observed in 11 patients, and oral hairy leukoplakia was observed in 2 patients. Candida species were isolated in 38 patients. Isolated Candida species were Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis. Scanning electron microscopic observation revealed a lot of slender thread-like hyphae of Candida albicans on the surface of the exfoliative cells and in the intercellular spaces. Numerous spherical yeast-like mycelium which were adherent to the surface of the epithelial cells were observed. Hyphe of Candida albicans were found in the cytoplasm of the epithelial cells in the stratum corneum with transmission electron microscopy.

我们调查了柏林洪堡大学Charite医院传染病科50例接受高效抗逆转录病毒治疗超过6个月的HIV血清阳性患者的口腔病变。患者包括42名男性和8名女性，平均年龄为41.5岁（24-61岁）。同性恋者29例，异性恋者6例，吸毒者7例，其他8例。CDC分型分布为A1型1例，A2型8例，A3型1例，B1型1例，B2型6例，B3型13例，C3型20例。27例患者观察到口腔念珠菌病，11例患者观察到口腔念珠菌病伴其他口腔病变，2例患者观察到口腔毛状白斑。38例患者分离出念珠菌。分离的念珠菌属为白色念珠菌、光滑念珠菌、克柔念珠菌、热带念珠菌。扫描电镜观察可见脱落细胞表面及细胞间隙有大量丝状白念珠菌菌丝。观察到大量球形酵母样菌丝体粘附在上皮细胞表面。透射电镜下可见角质层上皮细胞胞浆内有白色念珠菌菌丝。

项目成果

Kazumasa Sugihara: "AIDS"The Journal of The Japan Dental Association. Vol. 55-No. 11. 78-79 (2003)

杉原一正：“艾滋病”日本牙科协会杂志。

白川正順, 伊藤隆利, 河村 博 編集: "有病者歯科診療(分担執筆) 杉原一正:エイズ(AIDS)"医歯薬出版株式会社. 304(147-149) (2003)

白川正明、伊藤贵俊、河村宏（编）：《疾病患者的牙科治疗（撰稿人）杉原一正：艾滋病》石药出版株式会社 304（147-149）（2003）

白川正順, 伊東隆利, 河村 博 編: "有病者歯科診療(分担執筆)、杉原一正:エイズ(AIDS)"医歯薬出版株式会社. 304(147-149) (2001)

白川正明、伊藤隆俊、河村宏（编）：“疾病患者的牙科治疗（撰稿人）、杉原一正：艾滋病”石药出版有限公司 304（147-149）（2001）

杉原一正: "AIDS(エイズ)"日本歯科医師会雑誌. 55・11. 78-79 (2003)

杉原和正：《艾滋病》日本牙科协会杂志 55・11（2003 年）。

杉原一正: "AIDS(エイズ)"目本歯科医師会雑誌. 55・11. 78-79 (2003)

杉原一正：《艾滋病》梅本牙科协会杂志 55・11（2003 年）。