Electron microscopic study on oral lesions of HIV-infection

我们调查了在柏林洪堡大学夏利特医院感染科接受高效抗逆转录病毒治疗6个月以上的50例HIV血清阳性患者的口腔病变。男42例，女8例，平均年龄41.5岁(24~61岁)。同性恋者29例，异性恋者6例，吸毒者7例，其他8例。CDC分类分布A1 1例，A2 8例，A3 1例，B1 1例，B2 6例，B3 13例，C3 20例。口腔念珠菌病27例，口腔念珠菌病合并其他口腔病变11例，口腔毛状白斑2例。38例患者分离到念珠菌。分离出的念珠菌有白色念珠菌、光滑念珠菌、克鲁斯假丝酵母菌、热带念珠菌。扫描电子显微镜观察发现，脱落细胞表面和细胞间隙内有大量细长的白色念珠菌菌丝。可见大量球形酵母样菌丝附着在上皮细胞表面。透射电子显微镜下可见白色念珠菌在角质层上皮细胞胞浆内。

We investigated on oral lesions of 50 HIV-seropositive patients treated with highly active antiretroviral therapy for over 6 months at the Department of Infectious Disease in Charite Hospital, Humboldt University in Berlin. The patients consisted of 42 men and 8 women with a mean age of 41.5 years (24-61 years). 29 patients were homosexuals, 6 patients were heterosexuals, 7 patients were drug abusers, and 8 patients were others. Distribution of CDC-classification was 1 in A1, 8 in A2, 1 in A3, 1 in B1, 6 in B2, 13 in B3 and 20 in C3. Oral candidiasis was observed in 27 patients, oral candidiasis with other oral lesions was observed in 11 patients, and oral hairy leukoplakia was observed in 2 patients. Candida species were isolated in 38 patients. Isolated Candida species were Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis. Scanning electron microscopic observation revealed a lot of slender thread-like hyphae of Candida albicans on the surface of the exfoliative cells and in the intercellular spaces. Numerous spherical yeast-like mycelium which were adherent to the surface of the epithelial cells were observed. Hyphe of Candida albicans were found in the cytoplasm of the epithelial cells in the stratum corneum with transmission electron microscopy.