Redirecting the carbon flux by implementing energy-conserving modules in Methanothermobacter thermautotrophicus to capture carbon dioxide

通过在嗜热甲烷杆菌中实施节能模块来捕获二氧化碳来重定向碳通量

• 批准号: 536033891
• 负责人: Dr. Bastian Molitor
• 金额: --
• 依托单位: Arbeitsgruppe Umweltbiotechnologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/536033891?language=en
• 关键词: Redirecting; carbon; flux; implementing; energy

Renewable energy sources are well-established parts of the electricity mix for many countries. However, an imbalance between production and consumption makes storing surplus renewable electric power necessary. Hydrogen (H2) can be generated through the electrolysis of water with renewable energy. Using H2 to produce methane gas (CH4) from carbon dioxide (CO2) in a power-to-gas process with biomethanation has already become a solution to overcome the costly direct storage of H2. Pure cultures of thermophilic methanogenic archaea, such as Methanothermobacter thermautotrophicus, are applied to convert CO2 and H2 into CH4 with high process stability and production rates. The resulting renewable natural gas contains >97% CH4. With only little additional conditioning, this gas can be introduced into the existing natural gas grid, with vast storage capacity and great distribution possibilities. My lab has developed a genetic system for M. thermautotrophicus, which can now be utilized to harness the full potential of this biotechnology by investigating and optimizing the microbial physiology of the biocatalyst and broadening the product spectrum in a power-to-chemicals approach. Based on the available genetic system, we will develop additional tools, for example, to produce tagged enzyme variants in M. thermautotrophicus. This will allow us to study the biochemistry of highly relevant enzymes for methanogenesis. For this purpose, we will collaborate with partners in the US and Australia. The genetic tools, in combination with steady-state fermentation and systems biology data, will be exploited to optimize the metabolism of M. thermautotrophicus to produce acetoin as a proof-of-concept product. For example, acetoin is of economic value as a flavor enhancer, cosmetics ingredient, and precursor to further platform chemicals such as 2,3-butanediol. My lab has also developed a genome-scale metabolic model, which provides the platform to test hypotheses in silico before wet lab experiments. We have used the model to simulate metabolic changes that could lead to higher flux toward acetoin, which will be tested in this proposal. With this, we will demonstrate that M. thermautotrophicus can be utilized as a rigid microbial chassis to produce other biotechnologically relevant products in a power-to-chemicals platform.

可再生能源是许多国家电力结构的一部分。然而，生产和消费之间的不平衡使得储存剩余的可再生电力成为必要。氢气（H2）可以通过电解水与可再生能源产生。在具有生物甲烷化的电力制气工艺中，使用H2从二氧化碳（CO2）生产甲烷气体（CH 4）已经成为克服H2直接储存成本高的解决方案。纯培养的嗜热产甲烷古菌，如甲烷热细菌thermautotrophicus，应用于转换CO2和H2到CH 4具有高的过程稳定性和生产率。由此产生的可再生天然气含有>97%的CH 4。只需很少的额外调节，这种气体就可以引入现有的天然气电网，具有巨大的存储容量和巨大的分配可能性。我的实验室为M.热营养菌，现在可以通过研究和优化生物催化剂的微生物生理学并以电力到化学品的方法拓宽产品范围来利用这种生物技术的全部潜力。基于现有的遗传系统，我们将开发其他工具，例如，在M中产生标记的酶变体。热自养的这将使我们能够研究与甲烷生成高度相关的酶的生物化学。为此，我们将与美国和澳大利亚的合作伙伴合作。将利用遗传工具，结合稳态发酵和系统生物学数据，优化M.以产生乙偶姻作为概念验证产品。例如，乙偶姻作为增味剂、化妆品成分和其他平台化学品如2，3-丁二醇的前体具有经济价值。我的实验室还开发了一个基因组规模的代谢模型，它提供了在湿实验室实验之前测试假设的平台。我们已经使用该模型来模拟代谢变化，这可能导致更高的流向乙偶姻，这将在本提案中进行测试。我们将证明M。热自养菌可用作刚性微生物底盘，以在动力-化学品平台中生产其它生物技术相关产品。