Regeneration research of peripheral nerve injury via macrophage stimulated by oxidized galectin-1

氧化半乳糖凝集素1刺激巨噬细胞修复周围神经损伤的再生研究

• 批准号: 19591004
• 负责人: YOSHIMURA Kazunori
• 金额: $ 2.83万
• 依托单位: Nihon institute of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591004/
• 关键词: 神経病態生化学; 神経再生促進; マクロファージ; 神経栄養因子; プロテオミクス; 神経再生促進作用

We identified several kind of proteins related with signal transduction of oxidized galectin-1 in macrophages stimulated with oxidized galectin-1 by fluorescence two dimensional difference gel electrophoreis (2D DIGE). The proteins were arginase, GRP78, transaldolase, glia maturation factor, and malate dehydrogenase. We have been investigating about macrophages stimulated by oxidized galecin-1 in detail with Real Time RT-PCR, Western blot, Calcium Imaging, and proteome analysis.

我们通过荧光二维差异凝胶电泳（2D DIGE）鉴定了氧化半乳糖凝集素-1刺激的巨噬细胞中与氧化半乳糖凝集素-1信号转导相关的几种蛋白质。这些蛋白质是精氨酸酶、GRP78、转醛醇酶、神经胶质成熟因子和苹果酸脱氢酶。我们一直在通过实时 RT-PCR、蛋白质印迹、钙成像和蛋白质组分析详细研究氧化半乳糖蛋白-1 刺激的巨噬细胞。

项目成果

new monoclonal antibody, A3B10, specific for astrocyte-lineage cells recognizes calmodulin-regulated spectrin-associated protein 1

新型单克隆抗体 A3B10，专门针对星形胶质细胞谱系细胞，可识别钙调蛋白调节的血影蛋白相关蛋白 1

• 发表时间: 2009
• 期刊: J Neurosci Res 87(2)
• 作者: 山本雅浩;吉村和法;他
• 通讯作者: 他

Proteomic analysis of human astrocytes treated with propofol.

用异丙酚处理的人星形胶质细胞的蛋白质组学分析。

• 发表时间: 2007
• 作者: Toshiko Maruo;Kazunori Yoshimura;Kayo Fujimaki;Masahio Nomura;Shu-Ichi Watanabe;Masahiko Suzuki;Hideki Miyao
• 通讯作者: Hideki Miyao

Proteomic analysis of macrophages stimulated with oxidized galectin-1.

用氧化半乳糖凝集素 1 刺激的巨噬细胞的蛋白质组学分析。

• 发表时间: 2007
• 作者: Kazunori Yoshimura;Fuyuki Kametani;Kayo Fujimaki;Takashi Miyazaki;Hidenori Horie;Shu-Ichi Watanabe;Toshihiko Kadoya
• 通讯作者: Toshihiko Kadoya

Identification of casein kinase-1 phosphorylation sites on TDP-43

• DOI: 10.1016/j.bbrc.2009.03.038
• 发表时间: 2009-05-01
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kametani, Fuyuki;Nonaka, Takashi;Hasegawa, Masato
• 通讯作者: Hasegawa, Masato

Truncation and pathogenic mutations facilitate the formation of intracellular aggregates of TDP-43

• DOI: 10.1093/hmg/ddp275
• 发表时间: 2009-09-15
• 期刊: HUMAN MOLECULAR GENETICS
• 影响因子: 3.5
• 作者: Nonaka, Takashi;Kametani, Fuyuki;Hasegawa, Masato
• 通讯作者: Hasegawa, Masato