Isolation of neuroblastoma stem cell to compare with neural stem cell

我们曾计划从小鼠神经母细胞瘤细胞系中分离出可能的癌症干细胞。此外，我们还将从小鼠胚胎和成年脑中分离出小鼠神经干细胞。在分离这两种干细胞后，我们会发现神经母细胞瘤肿瘤干细胞和神经干细胞之间的相似和不同之处。我们已经分离出小鼠神经干细胞。然而，我们一直未能使用特定细胞表面标记的分选法或神经球法来分离神经母细胞瘤肿瘤干细胞。我们认为，经过频繁传代后，癌细胞株可能会失去人类原发肿瘤中存在的肿瘤干细胞。

We had a plan to isolate putative cancer stem cells from mouse neuroblastoma cell lines. And also we would isolate mouse neural stem cells from mouse embryonal and adult brain. Following the isolation of both stem cells, we would find the similarity and the difference between neuroblastoma cancer stem cells and neural stem cells. We have isolated mouse neural stem cells. However, we have failed to isolate neuroblastoma cancer stem cells using the sorting method by the specific cell surface marker or the neurosphere method. We suggest that after frequent passages, cancer cell lines could lose the cancer stem cells, which can be found in human primary tumor.