Anaiysis of microRNAs derived from human inrrahepatic cholangiocarcinoma cell lines

人肝内胆管癌细胞系来源的 microRNA 分析

• 批准号: 19591602
• 负责人: ISHIKAWA Tomoko
• 金额: $ 2.83万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591602/
• 关键词: 胆道外科学; 肝内胆管癌; microRNA; 胆道系悪性腫瘍; 胆管上皮; 低分子RNA; mRNA

肝内胆管癌由来細胞株で発現が消失または著明に減少している胆管上皮特異的microRNAを過剰発現させ、microRNA(miRNA)が制御している標的となるmRNAの同定を目的としたプロテォミクス解析を行った。この解析により、胆管上皮細胞特異的miRNAが、胆管上皮の癌化、肝内胆管癌の増殖と転移に関連するシグナル伝達経路の調節に関与することを示唆する新知見を得た。

The expression of microRNA specific to bile duct epithelium was reduced, and the expression of microRNA(miRNA) was reduced. New insights into the relationship between the analysis of miRNA specific to bile duct epithelial cells, the carcinogenesis of bile duct epithelium, and the proliferation and metastasis of intrahepatic cholangiocarcinoma and the regulation of bile duct pathways were obtained.

项目成果

MicroRNA (miRNA) cloning analysis reveals sex differences in miRNA expression profiles between adult mouse testis and ovary

• DOI: 10.1530/rep-08-0349
• 发表时间: 2008-12-01
• 期刊: REPRODUCTION
• 影响因子: 3.8
• 作者: Mishima, Takuya;Takizawa, Takami;Takizawa, Toshihiro
• 通讯作者: Takizawa, Toshihiro

MicroRNA profiling of human intrahepatic cholangiocarcinoma cell lines reveals biliary epithelial cell-specific microRNAs.

• DOI: 10.1272/jnms.76.188
• 发表时间: 2009-08
• 期刊: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi
• 作者: Yutaka Kawahigashi;T. Mishima;Y. Mizuguchi;Y. Arima;S. Yokomuro;Tomohiro Kanda;O. Ishibashi;H. Yoshida;T. Tajiri;T. Takizawa

MicroRNA Profiling of Human Intrahepatic Cholangiocarcinoma Lines Reveals Biliary Epithelial Cell-Specific MicroRNAs

人肝内胆管癌细胞系的 MicroRNA 分析揭示了胆道上皮细胞特异性的 MicroRNA

• 发表时间: 2009
• 期刊: Journal of Nippon Medical School 76(in press)
• 作者: Yutaka Kawahigashi;Takuya Mishima;Yoshiaki Mizuguchi;Yasuo Arima;Shigeki Yokomuro;Tomohiro Kanda;Osamu Ishibashi;Hiroshi Yoshida;Takashi Tajiri;Toshihiro Takizawa;Yutaka Kawahigashi
• 通讯作者: Yutaka Kawahigashi

ヒト正常胆管上皮細胞と肝内胆管癌細胞株におけるmicroRNAの発現プロファイル解析

人正常胆管上皮细胞和肝内胆管癌细胞系MicroRNA表达谱分析

• 发表时间: 2008
• 作者: 瀧澤俊広;川東豊、三嶋拓也、水口義昭、石橋宰、吉田寛、田尻孝;川東 豊
• 通讯作者: 川東 豊

RT-PCR-based analysis of microRNA (miR-1 and-124) expression in mouse CNS

• DOI: 10.1016/j.brainres.2006.11.035
• 发表时间: 2007-02-02
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Mishima, Takuya;Mizuguchi, Yoshiaki;Takizawa, Toshihiro
• 通讯作者: Takizawa, Toshihiro