The interaction between adipose tissue and tubular epithelium on their proliferation, polarization and differentiation

脂肪组织和管状上皮之间的相互作用对其增殖、极化和分化的影响

• 批准号: 19591858
• 负责人: UOZUMI Jiro
• 金额: $ 2.58万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591858/
• 关键词: 尿細管上皮; 再生医学; 脂肪細胞; 間葉系幹細胞; 細胞増殖・分化; 泌尿器科

Adipokine-producing fatty tissues, composed of preadipocytes, adipocytes, and mesenchymal stem cells, surround the kidney. To study the interaction between renal tubular cells and adipose tissue, we cocultured adipose tissue fragments and MDCK cells. MDCK cells in the coculture showed fine differentiation than that seen in MDCK cell monoculture. The adipose tissue-induced change in morphology was replicated when we added leptin to MDCK cells cultured alone. Our study shows that adipose tissue fragments promote the hypertrophy, polarization, and differentiation of MDCK cells by attenuating their growth and apoptosis through opposing endocrine or paracrine effects of leptin and adiponectin. Further, MDCK cells inhibit the regeneration of preadipocytes and mesenchymal stem cells in adipose tissue.

产生脂肪因子的脂肪组织，由前脂肪细胞、脂肪细胞和间充质干细胞组成，环绕在肾脏周围。为了研究肾小管细胞与脂肪组织的相互作用，我们将脂肪组织片段与MDCK细胞共培养。共培养的MDCK细胞比单培养的MDCK细胞分化较好。当我们在单独培养的MDCK细胞中加入瘦素时，脂肪组织诱导的形态学变化被复制。我们的研究表明，脂肪组织碎片通过对抗瘦素和脂联素的内分泌或旁分泌作用，通过抑制MDCK细胞的生长和凋亡，从而促进MDCK细胞的肥大、极化和分化。此外，MDCK细胞抑制脂肪组织中前脂肪细胞和间充质干细胞的再生。

项目成果

尿細管上皮細胞の増殖、分化に与える脂肪組織の影響

脂肪组织对肾小管上皮细胞增殖和分化的影响

• 发表时间: 2008
• 作者: Kazuma Udo;Shigehisa Aoki;Kazuyoshi Uchihashi;Maki Kawasaki;Aki Matsunobu;Yuji Tokuda;Akifumi Ootani;Shuji Toda;Jiro Uozumi;有働和馬;有働和馬;有働和馬
• 通讯作者: 有働和馬

脂肪組織は尿細管上皮の構造・機能分化を促進する

脂肪组织促进肾小管上皮的结构和功能分化

• 发表时间: 2008
• 作者: Kazuma Udo;Shigehisa Aoki;Kazuyoshi Uchihashi;Maki Kawasaki;Aki Matsunobu;Yuji Tokuda;Akifumi Ootani;Shuji Toda;Jiro Uozumi;有働和馬
• 通讯作者: 有働和馬

Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture.

脂肪组织外植体和 MDCK 细胞在共培养中相互调节其形态发生。

• 发表时间: 2010
• 期刊: Kidney Int. (in press)
• 作者: Kazuma Udo;Shigehisa Aoki;Kazuyoshi Uchihashi;Maki Kawasaki;Aki Matsunobu;Yuji Tokuda;Akifumi Ootani;Shuji Toda;Jiro Uozumi
• 通讯作者: Jiro Uozumi

尿細管上皮の増殖、分化に与える脂肪組織の影響

脂肪组织对肾小管上皮增殖和分化的影响

• 发表时间: 2008
• 作者: Kazuma Udo;Shigehisa Aoki;Kazuyoshi Uchihashi;Maki Kawasaki;Aki Matsunobu;Yuji Tokuda;Akifumi Ootani;Shuji Toda;Jiro Uozumi;有働和馬;有働和馬
• 通讯作者: 有働和馬