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UV-A特異的光センシングシステムの解明

UV-A 特定光学传感系统的阐明

基本信息

批准号：
19658012
负责人：
河鰭実之
金额：
$ 2.05万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

植物は青色光,UV-A,およびUV-Bに応答してアントシアニンを合成する.カルコン合成酵素(CHS)はアントシアニン生合成経路の鍵酵素である.トマトではCHS1とCHS2の2種類が単離されており,カブからは今年度の研究により計6種類のCHSが単離できた.これらの発現を半定量RT-PCRまたはリアルタイムPCRにより調べた.野生型およびクリプトクロム1欠失突然変異体(cry1)のトマト,およびカブ'津田カブ'を暗黒下で発芽させ,青色光,UV-B,UV-A,またはUV-B+青を24時間照射した.トマトとカブのいずれでも,青により下胚軸上部に,UV-Aにより下胚軸中下部にアントシアニンが蓄積した.青を照射したとき,野生型トマトでは下胚軸上部のCHS1の発現が高く,cry1ではCHS1の発現はほとんど見られなかった.一方,CHS2は,野生型,cry1のいずれでもUV-Aを照射したときに下胚軸中下部で発現が見られた.このことは,クリプトクロムが光を感知した場合にはCHS1がはたらき,これとは別にUV-Aを感知してCHS2の発現を誘導する系が存在することを示した.カブにおいては,CHS1,CHS5がUV-Aにより,またCHS4がUV-B+青により下胚軸中下部で強い発現を示した.さらに,転写調節因子であるMYBファミリーのうちPAP1の発現は,青やUV-B+青では下胚軸上部で発現が誘導され,UV-A照射では下胚軸下部における発現が高まった.このようにPAP1の発現パターンはアントシアニン蓄積パターンとよく一致した.また,MYB12はUV-AおよびUV-B+青により,MYB111はUV-B+青により発現が誘導された.以上により異なる波長の光によるアントシアニン合成の誘導においては異なるCHSが働き,その調節にMYBが関与していることが推測された.

The green light of the plant, UV-A, UV-B, answers the question that the plant synthesizes. The synthetic enzyme (CHS) was isolated from the synthetic enzyme. In this year's study, there are six categories of CHS1 and CHS2 in this year's study, and there are six categories of CHS in this year. It is found that semi-quantitative RT-PCR is available because of the low cost of PCR. In the wild type, there was a sudden loss of body mass (cry1), and the green light, green light, UV-B,UV-A, green, green In the middle and lower part of the hypoembryo, the lower part of the lower embryo, the lower part of the UV-An embryo, the lower part of the lower embryo, the lower part of the embryo. In the wild type, the upper part of the embryo is CHS1, and the upper part of the embryo is higher than that of the wild type, and the upper part of the lower embryo of the wild type is high in the upper part of the embryo, while that in the upper part of the embryo is higher than that in the upper part of the embryo, while that in the upper part of the lower embryo is higher than that in the upper part of the embryo, and that in the upper part of the embryo is higher than that in the upper part of the embryo, and that in the upper part of the lower embryo is higher than that in the upper part of the embryo, and that in the upper part of the embryo is higher than that in the upper part of the embryo, and that in the upper part of the embryo, the upper part of the lower embryo, the upper part of the CHS1, the upper part of the embryo, the upper part of the embryo, the upper On one side, CHS2, wild type, cry1, wild type, cryophora, cryopodium, cryophora, cryopodium, cryopodium. This is not true because of the presence of light perception, CHS1 awareness, CHS2 awareness, CHS2 awareness, light perception, light perception, light perception The lower part of the embryo is strongly displayed in the middle and lower part of the embryo, which means that the lower part of the embryo is strongly displayed in the middle and lower part of the embryo, CHS1,CHS5, CHS4, UV-B+, and the lower part of the embryo. In this study, the factors were detected by MYB. The upper part of the hypodermis of UV-B+ was detected, and the lower part of the hypodermis was irradiated by UV-A. Please tell me that the PAP1 is in good agreement with each other. MYB111, MYB12, UV-A, UV-B+, MYB111, UV-B+, and so on. The above optical wavelengths are sensitive to photoluminescence, and the synthesis of optical fiber leads to the detection of CHS signals, and the response of MYB sensors and infrared sensors to improve the performance.