植物細胞サイズの転写制御

植物细胞大小的转录控制

• 批准号: 25840112
• 负责人: BREUER Christian
• 金额: $ 2.91万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25840112/
• 关键词: 発生・分化

In order to understand the molecular function of the GT-2 type trihelix transcription factor family for cell differentiation and organogenesis, I have carried out cell type-specific transcriptional profiling and genome-wide chromatin binding assays in Arabidopsis roots. The data analysis of these large data sets clearly show an enrichment of genes which belong to distinct hormonal signaling pathways. In addition, I was able to confirm the preliminary results directly in Arabidopsis plants using representative molecular markers in combinations of loss-of-function alleles of those gene family members. In the future, I will further investigate this novel functional link using genetic, physiological and molecular approaches.

为了了解GT-2型三螺旋转录因子家族在细胞分化和器官发生中的分子功能，我在拟南芥根中进行了细胞类型特异性转录谱分析和全基因组染色质结合分析。这些大数据集的数据分析清楚地显示了属于不同激素信号传导途径的基因的富集。此外，我能够直接在拟南芥植物中使用这些基因家族成员的功能丧失等位基因的组合中的代表性分子标记来确认初步结果。在未来，我将进一步研究这种新的功能链接使用遗传，生理和分子方法。