Development of an automatic analysis system at the protein-structural mobility using the electron microscope

使用电子显微镜开发蛋白质结构迁移率自动分析系统

• 批准号: 20300107
• 负责人: OGURA Toshihiko
• 金额: $ 9.32万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20300107/
• 关键词: 電子顕微鏡; 画像情報処理; 生物サンプル; 3次元構造; タンパク質; 生物試料; 構造変化; 並列分散処理

To reconstruct three-dimensional(3D) structure of proteins, we have developed newly 3D reconstruction algorithm and a high-contrast detection system of the biological samples. Our 3D reconstruction method was composed with the modified Simulated-Annealing algorithm with the high accuracy classification method of the class averages from the protein images by the transmission-electron microscopy. This method enables automatically 3D reconstruction from the protein images. Furthermore, we developed high-contrast and low-damage 3D detection system. This system contains a linear array PD system and new 3D reconstruction method.

为了重建蛋白质的三维结构，我们开发了新的三维重建算法和高对比度的生物样品检测系统。我们的三维重建方法是由透射电子显微镜蛋白质图像的类平均值的高精度分类方法与改进的模拟退火算法组成。该方法能够从蛋白质图像自动进行3D重建。此外，我们开发了高对比度和低损伤的三维检测系统。该系统包括一个线阵PD系统和新的三维重建方法。

项目成果

Measurement of the unstained biological sample by a novel scanning electron generation X-ray microscope based on SEM

• DOI: 10.1016/j.bbrc.2009.05.107
• 发表时间: 2009-08-07
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ogura, Toshihiko

Reconstruction of the P2X2 receptor reveals a vase-shaped structure with lateral tunnels above the membrane

P2X2 受体的重建揭示了膜上方有横向隧道的花瓶状结构

• DOI: 10.1016/j.str.2008.12.007
• 发表时间: 2009
• 期刊: Structure
• 影响因子: 5.7
• 作者: Kazuhiro Mio;Toshihiko Ogura;Tomomi Yamamoto;Yoko Hiroaki;Yoshinori Fujiyoshi;Yoshihiro Kubo and Chikara Sato
• 通讯作者: Yoshihiro Kubo and Chikara Sato

Direct observation of unstained wet biological samples by scanning-electron generation X-ray microscopy

• DOI: 10.1016/j.bbrc.2009.11.031
• 发表时间: 2010-01-01
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ogura, Toshihiko

Analyzing indirect secondary electron contrast of unstained bacteriophage T4 based on SEM images and Monte Carlo simulations

• DOI: 10.1016/j.bbrc.2009.01.046
• 发表时间: 2009-03-06
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ogura, Toshihiko

A high contrast method of unstained biological samples under a thin carbon film by scanning electron microscopy

• DOI: 10.1016/j.bbrc.2008.09.097
• 发表时间: 2008-12-05
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Ogura, Toshihiko