Development of an automatic analysis system at the protein-structural mobility using the electron microscope

为了重建蛋白质的三维结构，我们开发了新的三维重建算法和生物样品的高对比度检测系统。我们的三维重建方法是由改进的模拟退火法和高精度的类平均分类方法组成的。这种方法可以从蛋白质图像中自动进行三维重建。此外，我们还开发了高对比度、低损伤的三维探测系统。该系统包括线阵局部放电系统和新的三维重建方法。

To reconstruct three-dimensional(3D) structure of proteins, we have developed newly 3D reconstruction algorithm and a high-contrast detection system of the biological samples. Our 3D reconstruction method was composed with the modified Simulated-Annealing algorithm with the high accuracy classification method of the class averages from the protein images by the transmission-electron microscopy. This method enables automatically 3D reconstruction from the protein images. Furthermore, we developed high-contrast and low-damage 3D detection system. This system contains a linear array PD system and new 3D reconstruction method.