Dynamic Manipulation of Molecules Embedded within Artificial Cell Membrane Supported on Solid Surface

在本计画中，我们的目标是建立一种利用局部电场作用于支撑脂质双分子层（SLB）来操控分子的技术。固-液界面上的支撑脂质双层保持了横向扩散的特性，因此可以看作是一个二维分子系统。我们取得了以下两项重大成就。(1)我们已经成功地形成了染料共轭脂分子的梯度内SLB使用的固体基板装备的掩埋电极。这意味着由电场引起的力克服了双层中的布朗运动。(2)我们已经成功地控制了自蔓延的开关电压提供给一对电极与纳米间隙的距离。这意味着在纳米间隙位置处的电场对分子的捕获力克服了用于驱动自扩散的能量。利用纳米间隙作为“分子门”，实现了对自展开脂双层宏观结构的动态控制。

In this project, we aimed at establishing the technique for manipulating molecule(s) embedded within a supported lipid bilayer (SLB) using electric field locally applied to the bilayer. The supported lipid bilayer at solid-liquid interface maintains the lateral diffusion characteristics, thus can be regarded as a 2-dimensional molecule system for the embedded molecule(s). We reached to the following two significant achievements. (1) We have successfully formed the gradation of dye-conjugated lipid molecules within SLB using the solid substrate equipping the buried electrodes. This means that the force induced by the electric field overcomes the Brownian motion in the bilayer. (2) We have successfully controlled the self-spreading by switching on and off the voltage supplied to a pair of electrodes with nanogap distance. This means that the trapping force of molecules by an electric field at the nanogap position overcomes the energy for driving self-spreading. By using the nanogap as "molecule gate", we have achieved the dynamic control of the macroscopic structure of self-spreading lipid bilayer.