Manipulation of chicken primordial germ cells and its application to producing transgenic chickens

为了开发生产转基因鸡的技术，我们尝试从早期胚胎中分离原始生殖细胞进行体外长期培养。培养的原始生殖细胞在移植到受体胚胎中后保留了迁移到性腺的能力，并且还证实了在受体性腺中分化为功能配子的能力。将GFP基因导入培养的原始生殖细胞中，培养的表达GFP基因的原始生殖细胞成功进入受体胚胎的性腺。

In order to develop a technique producing transgenic chickens, we attempted to culture primordial germ cells isolated from early-stage embryos in vitro for long-term. The cultured primordial germ cells retained the ability to migrate to gonads after transfer into recipient embryos and also confirmed the ability of differentiating to functional gametes in recipient gonads. Introduction of GFP gene into the cultured primordial germ cells was performed and cultured primordial germ cells expressing GFP gene were successfully entered gonads of recipient embryos.