Development of enrichment methods of sialoglycopeptides towards glycobiomarker discovery

糖蛋白作为新型临床生物标志物具有很大的潜力。我们研究了一种新的唾液糖蛋白富集方法，该方法在其糖基上含有唾液酸，作为发现唾液糖蛋白生物标志物的一种方法。经过大量的试验，我们发现肟形成是涎糖蛋白与树脂珠偶联最实用的方法之一。然后，我们设计并合成了一种可以在唾液酸部分引入氧化胺基的连接体。

Glycoproteins have great potential as novel clinical biomarkers. We studied on the development of a novel enrichment method of sialoglycoproteins which contain sialic acids on their glycan moieties, as an approach to biomarker discovery focused on sialoglycoproteins. After intensive trials, we found that oxime formation is one of the most practical methods for coupling of sialoglycoproteins and resin beads. Then, we designed and synthesis of a linker which can introduce oxyamine group at sialic acid moiety.