The role of Sox17 during mouse early endoderm to its delivertives

最终的肠道内胚层产生各种细胞类型，包括消化道、肝脏、胰腺和相关的内脏内胚层。为了检查原基内胚层(9)。5 dpc)发育，培养3天，然后分析每个外植体的发育/成熟。我们研究了Sox17空ES细胞导入野生胚泡及其DNA阵列之间的嵌合研究。我们的观察结果强烈表明，Sox17在哺乳动物细胞进入内胚层及其衍生器官的决定中起着关键作用。这些发现提示sox17可能是未来人类治疗性研究中分离内胚层干细胞和获得胚胎干细胞衍生内胚层细胞的关键基因之一。

The definitive gut endoderm give rise to various cell-types constituting digestive tract, liver, pancreas and associated visceral endoderm. In order to examine primordium endoderm(9. 5 dpc) development, we have cultured them for 3 days, and then analyzed the development/maturation in each explant. We have examined that chimeric study between Sox17 null ES cells introducing to wild blastcyst and their DNA array. Our observation strongly indicate that a critical role of Sox17 in the determination of the cell into the endoderm and its derivative organ in mammals. These findings lead us to the possibility that Sox17is one of the key genes for the isolation of the endodermal stem cells and obtaining of the endodermal cells derived from ES cells in the human therapeutic research for the future.