Identification of regulatory factors for genomic imprinting using siRNA library

使用 siRNA 文库鉴定基因组印记的调控因子

• 批准号: 20590330
• 负责人: SOEJIMA Hidenobu
• 金额: $ 3万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590330/
• 关键词: エピジェネティクス; ゲノム刷り込み; 刷り込みドメイン; non-coding RNA; siRNAライブラリー; KIP2; LIT1刷り込みドメイン

The aim of this study is to clarify regulatory mechanisms of the imprinting. (1) To distinguish imprinting disrupted cells from normal cells by expression of marker genes, Igf2r was targeted with YFP cassette on the maternal allele in F1ES cell. We are trying to introduce Igf2r-2A-E2Crimsoncassette into the paternal allele. The cells will be screened with siRNA library to identify regulatory factors for the imprinting. (2) non-coding RNA LIT] would recruit HDAC(s) to the promoter region of KvLQTl to deacetylate histone H3 and to make heterochromatin, leading to repress the KvLQTl gene on the paternal chromosome. (3) At mouse Commdl/U2af1-rsl imprinted domain, that interference with paternal Commdl transcription by the oppositely directed U2afl-rs1 transcription seemed to be a mechanism for the maternal predominantly expression of Commdl gene.

本研究的目的是阐明印记的调控机制。(1)为了通过标记基因的表达将印迹破坏的细胞与正常细胞区分开，在F1 ES细胞中用YFP盒靶向Igf 2 r的母体等位基因。我们正在尝试将Igf 2 r-2A-E2基因盒导入父系等位基因。将用siRNA文库筛选细胞以鉴定印记的调节因子。(2)非编码RNA LIT]将募集HDAC至KvLQT 1的启动子区以使组蛋白H3脱乙酰化并产生异染色质，从而导致抑制父本染色体上的KvLQT 1基因。(3)在小鼠Commdl/U2 afl-rsl印迹结构域，U2 afl-rsl转录对父本Commdl转录的干扰似乎是Commdl基因的母本优势表达的机制。

项目成果

Antisense transcription occurs at the promoter of a mouse imprintedgene, Commdl, on the repressed paternal allele.

反义转录发生在受抑制的父本等位基因上的小鼠印记基因 Commdl 的启动子处。

• 发表时间: 2009
• 期刊: The Journal of Biochemistry 146
• 作者: 宮川卓;他;Joh Keiichiro
• 通讯作者: Joh Keiichiro

Organotypic culture of human bone marrow adipose tissue for analyzing its biological roles.

人骨髓脂肪组织的器官型培养，用于分析其生物学作用。

• 发表时间: 2010
• 期刊: Pathol Int 60(4)
• 作者: Uchihashi K;Soejima H;Toda S
• 通讯作者: Toda S

特集小児疾患における臨床遺伝学の進歩Beckwith-Wiedemann症候群

儿科疾病贝克威斯-维德曼综合征临床遗传学的特色进展

• 发表时间: 2009
• 期刊: 小児科 50
• 作者: Joh K;Yatsuki H(equal contribution);Super Science High School Consortium;副島英伸;東元健
• 通讯作者: 東元健

A new organotypic culture of adipose tissue fragments maintains viable mature adipocytes for a long term, together with development of immature adipocytes and mesenchymal stem cell-like cells

• DOI: 10.1210/en.2008-0525
• 发表时间: 2008-10-01
• 期刊: ENDOCRINOLOGY
• 影响因子: 4.8
• 作者: Sonoda, Emiko;Aoki, Shigehisa;Toda, Shuji
• 通讯作者: Toda, Shuji

ADH1BおよびALDH2における琉球諸島vs北部九州ヒト集団の比較遺伝学解析

琉球群岛与北九州人群 ADH1B 和 ALDH2 的比较遗传分析

• 发表时间: 2009
• 作者: 小金渕佳江;松草博隆;中込滋樹;石田肇;埴原恒彦;副島英伸;藤本一眞;河村正二;太田博樹
• 通讯作者: 太田博樹