Improvement in the efficacy of cardiogenesis by Wnt-3a in mouse embryonic stem cells

Wnt-3a 改善小鼠胚胎干细胞的心脏生成功效

• 批准号: 20590410
• 负责人: UENO Shuichi
• 金额: $ 3万
• 依托单位: Jichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590410/
• 关键词: 胚性幹細胞(ES細胞); 再生医学; 発生・分化; 心筋細胞; Wnt; 胚性肝細胞(ES細胞)

The transient inhibition of TGF-beta/activin signaling at the initiation of cardiac differentiation increases Wnt-3a-induced cardiogenesis in mouse ES cells. In the following phase, simultaneous stimulation of activin A and Wnt-3a also enhances cardiac differentiation.The transient inhibition of BMPs signaling is more critical for the differentiation into cardiac myocytes induced by Wnt-3a than the activation of BMPs signaling.I propose that controlling TGF-beta/activin and BMP signaling will improve Wnt-3a-induced cardiogenesis in mouse ES cells.

在心脏分化开始时短暂抑制 TGF-β/激活素信号会增加小鼠 ES 细胞中 Wnt-3a 诱导的心脏发生。在接下来的阶段，同时刺激激活素A和Wnt-3a也会增强心脏分化。对于Wnt-3a诱导的心肌细胞分化，BMPs信号传导的瞬时抑制比BMPs信号传导的激活更为关键。我认为控制TGF-β/激活素和BMP信号传导将改善Wnt-3a诱导的小鼠ES细胞的心脏发生。

项目成果

Inhibition of BMP-2/4 Signaling Combined with Wnt-3a Stimulation Enhances the Differentiation into Cardiac Myocytes in Mouse Embryonic Stem Cells.

抑制 BMP-2/4 信号传导与 Wnt-3a 刺激相结合可增强小鼠胚胎干细胞向心肌细胞的分化。

• 发表时间: 2010
• 作者: Shuichi Ueno;Kazuyuki Shimada;Kazuomi Kario
• 通讯作者: Kazuomi Kario

Activin A Enhances the Differentiation into Cardiac Myocytes Accompanied with Wnt-3a in Mouse Embryonic Stem Cells.

激活素 A 促进小鼠胚胎干细胞中 Wnt-3a 向心肌细胞的分化。

• 发表时间: 2009
• 作者: Sumida Y;Kanemasa K;Yasui K;et al. (11名中8番目);Shuichi Ueno
• 通讯作者: Shuichi Ueno

Stage specific effect of TGF-beta/activin signaling in cardiogenesis induced by canonical Wnt signaling.

TGF-β/激活素信号传导在经典 Wnt 信号传导诱导的心脏发生中的阶段特异性作用。

• 发表时间: 2010
• 作者: Shuichi Ueno;Kazuyuki Shimada;Kazuomi Kario
• 通讯作者: Kazuomi Kario

Inhibition of TGF-beta/Activin Signaling in the Early Phase or Stimulation of Activin Signaling in the Following Phase Combined with Wnt-3a Stimulation Enhances the Differentiation into Cardiac Myocytes in Mouse Embryonic Stem Cells.

早期抑制 TGF-β/激活素信号传导或在后续阶段刺激激活素信号传导与 Wnt-3a 刺激相结合，可增强小鼠胚胎干细胞向心肌细胞的分化。

• 发表时间: 2010
• 作者: Shuichi Ueno;Kazuyuki Shimada;Kazuomi Kario
• 通讯作者: Kazuomi Kario

Biphasic Effect of TGF-b/Activin Signaling in Cardiogenesis Induced by Canonical Wnt Signaling.

TGF-b/激活素信号传导在规范 Wnt 信号传导诱导的心脏发生中的双相效应。

• 发表时间: 2010
• 作者: Shuichi Ueno;Kazuyuki Shimada;Kazuomi Kario
• 通讯作者: Kazuomi Kario