Development of a culture system to selectively induceγ δT-lymphocytes from embryonic and hematopoietic stem cells

开发从胚胎干细胞和造血干细胞中选择性诱导γδT淋巴细胞的培养系统

• 批准号: 20590491
• 负责人: KISHIHARA Kenji
• 金额: $ 3.08万
• 依托单位: Nagasaki International University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590491/
• 关键词: 自然免疫; γδT細胞; 幹細胞; γδTリンパ球; 胚性幹細胞; 造血幹細胞

In order to develop a culture system that can selectively induce the murine gd T lymphocyte differentiation, first, the gene expression of MHC class Ib and its related molecules as potential ligands of gd T lymphocytes in several stromal cell strains were quantitatively analyzed. From the results, stromal cell strains overexpressing T10 and T22 antigens as a first choice were established. On a single layer of the stromal cells, T lymphocytes were induced from embryonic stem cells but it has not observed the fact that T10 and T22 can induce the selective and predominant differentiation of gd T lymphocytes and influence their repertoire so far. The culture system is still being further analyzed.

为了建立能选择性诱导小鼠gd T淋巴细胞分化的培养体系，首先定量分析了几种基质细胞株中作为gd T淋巴细胞潜在配体的MHC类Ib及其相关分子的基因表达情况。根据实验结果，建立了过表达T10和T22抗原的基质细胞株作为首选。在单层基质细胞上，用胚胎干细胞诱导T淋巴细胞，但迄今尚未观察到T10和T22能诱导gd T淋巴细胞的选择性和显性分化并影响其库。文化体系还在进一步分析中。

项目成果

Notch signaling drives IL-22 secretion in the CD4+ T cells by stimulating the aryl hydrocarbon receptor

Notch 信号通过刺激芳烃受体驱动 CD4 T 细胞中 IL-22 的分泌

• 发表时间: 2010
• 期刊: Proceedings of the National Academy of Sciences U.S.A.
• 作者: 大隈貞嗣;緒方正人;M.S.Alam
• 通讯作者: M.S.Alam

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Essential Role of IL-17A in the Formation of a Mycobacterial Infection-Induced Granuloma in the Lung

• DOI: 10.4049/jimmunol.0903332
• 发表时间: 2010-04-15
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Yoshida, Yuko Okamoto;Umemura, Masayuki;Matsuzaki, Goro
• 通讯作者: Matsuzaki, Goro