Development of a culture system to selectively induceγ δT-lymphocytes from embryonic and hematopoietic stem cells

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In order to develop a culture system that can selectively induce the murine gd T lymphocyte differentiation, first, the gene expression of MHC class Ib and its related molecules as potential ligands of gd T lymphocytes in several stromal cell strains were quantitatively analyzed. From the results, stromal cell strains overexpressing T10 and T22 antigens as a first choice were established. On a single layer of the stromal cells, T lymphocytes were induced from embryonic stem cells but it has not observed the fact that T10 and T22 can induce the selective and predominant differentiation of gd T lymphocytes and influence their repertoire so far. The culture system is still being further analyzed.