Study for regeneration of function in neonatal hypoxic-ischemic encephalopathy using animal model of slowly progressive brain damage in rats

利用大鼠缓慢进行性脑损伤动物模型研究新生儿缺氧缺血性脑病的功能再生

• 批准号: 20590552
• 负责人: MISHIMA Kenichi
• 金额: $ 3万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590552/
• 关键词: 新生児; 低酸素性虚血性脳症; 緩徐進行性脳損傷; 機能再生; 性差; 脳虚血; 学習・記憶; 再生; 卵膜由来間葉系幹細胞; 細胞治療

We recently reported that rehabilitative training tasks during adolescence improve spatial learning impairment following neonatal hypoxic-ischemic (HI) injury in rats without histological improvement. In the present study we focused on sex differences. Wister rat pups were exposed to a unilateral hypoxic-ischemic insult at 7 days of age. Six weeks after hypoxia-ischemia, rehabilitative training tasks were started. The tasks consisted of the plus maze, the eight-arm radial maze, and the choice reaction time task. Sixteen weeks after the HI insult, the water maze task was performed to evaluate spatial learning ability. Afterwards, we morphologically examined brain injury. Our rehabilitative training significantly improved swimming time and length in females but not in males. Likewise, the training ameliorated infarct areas in the injured cerebral hemisphere in females but not in males. These results suggest that it may be important to develop and evaluate cognitive rehabilitation programs for children with brain injury on the basis of gender.

我们最近报道，在青春期的康复训练任务，改善空间学习障碍新生儿缺氧缺血性（HI）损伤大鼠没有组织学改善。在本研究中，我们主要关注性别差异。Wister大鼠幼鼠在7日龄时暴露于单侧缺氧缺血性损伤。缺氧缺血6周后开始康复训练任务。任务包括十字迷宫、八臂迷宫和选择反应时任务。HI损伤后16周，进行水迷宫任务以评估空间学习能力。之后，我们对脑损伤进行了形态学检查。我们的康复训练显着改善了女性的游泳时间和长度，但对男性没有影响。同样，训练可以改善女性受伤大脑半球的梗塞区域，但对男性却没有影响。这些结果表明，它可能是重要的，以性别为基础的脑损伤儿童的认知康复计划的开发和评估。

项目成果

Estradiol suppresses slowly progressive brain damage with learning impairment induced by hypoxia-ischemic in neonatal rats

雌二醇抑制新生大鼠缺氧缺血引起的缓慢进行性脑损伤和学习障碍

• 发表时间: 2009
• 作者: Sakamoto Y.;et al.
• 通讯作者: et al.

Cannabidiol prevents a post-ischemic injury progressively induced by cerebral ischemia via a high-mobility group box1-inhibiting mechanism

• DOI: 10.1016/j.neuropharm.2008.06.040
• 发表时间: 2008-12-01
• 期刊: NEUROPHARMACOLOGY
• 影响因子: 4.7
• 作者: Hayakawa, Kazuhide;Mishima, Kenichi;Fujiwara, Michihiro
• 通讯作者: Fujiwara, Michihiro

Inhibition of reactive astrocytes with fluorocitrate retards neurovascular remodeling and recovery after focal cerebral ischemia in mice

• DOI: 10.1038/jcbfm.2009.257
• 发表时间: 2010-04-01
• 期刊: JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
• 影响因子: 6.3
• 作者: Hayakawa, Kazuhide;Nakano, Takafumi;Fujiwara, Michihiro
• 通讯作者: Fujiwara, Michihiro

母乳由来の脂質およびその誘導体を有効成分とする抗菌ペプチドの発現誘導剤あるいは産生促進剤

含有母乳来源的脂质及其衍生物作为活性成分的抗菌肽表达诱导剂或产生促进剂

• 发表时间: 2009

Sex differences in the benefits of rehabilitative training during adolescence following neonatal hypoxia-ischemia in rats

• DOI: 10.1016/j.expneurol.2010.09.002
• 发表时间: 2010-12-01
• 期刊: EXPERIMENTAL NEUROLOGY
• 影响因子: 5.3
• 作者: Tsuji, Masahiro;Aoo, Naoya;Fujiwara, Michihiro
• 通讯作者: Fujiwara, Michihiro