Functional analysis of phosphoprotein to control endosperm formation and sugar metabolism in plant seed.

磷蛋白控制植物种子胚乳形成和糖代谢的功能分析。

• 批准号: 20780069
• 负责人: HAMADA Shigeki
• 金额: $ 2.66万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780069/
• 关键词: 植物生化学; リン酸化タンパク質; プロテインキナーゼ; デンプン生合成; Nucleooside diphosphate kinase 1; Heat shock protein 82; yeast two-hybrid system

Protein phosphorylation plays a key regulatory role in a variety of cellular processes. To better understand the function of protein phosphorylation in seed maturation, I employed a PCR-based cloning method and isolated five cDNA clones (pvcipk1-5) for protein kinases and two cDNA clones (pvcbl1 and 2) for CBLs from a cDNA library prepared from immature seeds of kidney bean (Phaseolus vulgaris L.). Yeast two-hybrid assay indicated that among the five PvCIPKs, only PvCIPK1 interacts with both PvCBL1 and PvCBL2. These results suggest that calcium-dependent protein phosphorylation-signaling via CBL-CIPK complexes occurs during seed development. Furthermore I identified heat shock protein 82 (Hsp82) and nucleooside diphosphate kinase 1 (NDPK1) as phosphoproteins in rice developing seed. The results of subcellular localization, gene expression and mutant analysis of these proteins indicated that Hsp82 and NDPK1 are involved in starch biosynthesis in plat seed.

蛋白质磷酸化在多种细胞过程中起着关键的调节作用。为了更好地了解蛋白质磷酸化在种子成熟中的作用，我采用了基于PCR的克隆方法，从菜豆（Phaseolus vulgaris L.）未成熟种子制备的cDNA文库中分离出5个蛋白激酶的cDNA克隆（pvcipk 1 -5）和2个CBL的cDNA克隆（pvcbl 1和2）。酵母双杂交实验表明，在5种PvCIPK中，只有PvCIPK 1能与PvCBL 1和PvCBL 2相互作用。这些结果表明，钙依赖性蛋白磷酸化信号通过CBL-CIPK复合物发生在种子发育过程中。此外，我还鉴定了热休克蛋白82（Hsp 82）和核苷二磷酸激酶1（NDPK 1）是水稻发育种子中的磷蛋白。亚细胞定位、基因表达和突变体分析结果表明，Hsp 82和NDPK 1参与了植物种子淀粉的合成。

项目成果

トウモロコシゼインの細胞内RNA局在に関与するシス制御配列の解析とRNA局在を利用したイネ胚乳細胞におけるタンパク質局在および蓄積量制御の試み

分析参与玉米醇溶蛋白细胞内 RNA 定位的顺式调控序列，并尝试利用 RNA 定位控制水稻胚乳细胞中的蛋白质定位和积累

• 发表时间: 2009
• 作者: Wang;C.;Washida;H.;Crofts;A.J.;Hamada;S.;Katsube-Tanaka;T.;Kim;D.;Choi;S.B.;Modi;M.;Shigh;S.;Okita;T. W;Changlin Wang et.al.;鷲田治彦
• 通讯作者: 鷲田治彦

The cytoplasmic-localized, cytoskeletal-associated RNA binding protein OsTudor-SN: evidence for an essential role in storage protein RNA transport and localization.

• DOI: 10.1111/j.1365-313x.2008.03516.x
• 发表时间: 2008-08
• 期刊: The Plant journal : for cell and molecular biology
• 作者: Changlin Wang;H. Washida;A. J. Crofts;S. Hamada;T. Katsube-Tanaka;Dongwook Kim;Sang-Bong Choi;M. Modi;Salvinder Singh;T. Okita

Expression and identification of the CBLs and CIPKs from immature see ds of kidney bean (Phaseolus vulEaris L.)

菜豆未成熟种子中CBLs和CIPKs的表达及鉴定

• 发表时间: 2009
• 期刊: Phytochemistry 70
• 作者: Washida;H.;Sugino;A.;Kaneko;S.;Crofts;N.;Sakulsingharoj;C.;Kim;D.;Choi;S.B.;Hamada;S.;Ogawa;M.;Wang;C.;Esen;A.;Higgins;T.J.;Okita;T. W;Washida Haruhiko et al.;Yoshiya Seto et.al.;Shigeki Hamada et.al.
• 通讯作者: Shigeki Hamada et.al.

イネ登熟種子由来リン酸化タンパク質Hsp82およびNDPK1の発現特性

成熟水稻种子磷酸化蛋白Hsp82和NDPK1的表达特征

• 发表时间: 2009
• 作者: Wang;C.;Washida;H.;Crofts;A.J.;Hamada;S.;Katsube-Tanaka;T.;Kim;D.;Choi;S.B.;Modi;M.;Shigh;S.;Okita;T. W;Changlin Wang et.al.;鷲田治彦;濱田茂樹
• 通讯作者: 濱田茂樹

Metabolism of tuberonic acid (12-hydroxyjasmonic acid) in plants: Purification and characterization of tuberonic acid glucosyltransferase activated by wounding stress

植物中块菌酸（12-羟基茉莉酸）的代谢：创伤应激激活的块菌酸葡萄糖基转移酶的纯化和表征

• 发表时间: 2009
• 期刊: Phytochemistry 70(3)
• 作者: Seto;Y.;Hamada;S.;Matsuura;H.;Satou;C.;Masuta;C.;Matsushige;M.;Takahashi;K.;Ito;H.;Matsui;H.;Nabeta;K
• 通讯作者: K