Establishment of mouse model of human cryoglobulinemic glomerulonephritis

人冷球蛋白血症性肾小球肾炎小鼠模型的建立

• 批准号: 20790590
• 负责人: ITO Kiyoaki
• 金额: $ 2.41万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790590/
• 关键词: クリオグロブリン; 糸球体腎炎; ハイブリドーマ; モデルマウス; リウマトイド因子; モノクローナルRF; 管内性増殖性腎炎; メサンギウム増殖性腎炎

The purpose of our study is to establish a mouse model of cryoglobulinemic (Cryo) glomerulonephritis (GN). We established the hybridoma cell line producing IgM-kappa cryoglobulin with rheumatoid factor activity (Cryo+IgM RF) from a patient with Cryo GN. We administered Cryo+IgM RF to wild type Balb/c mice. Urinary albumin increased. Pathological analysis of kidney tissue showed mild endocapillary proliferation, mild mesangial cell proliferation, and deposition of Cryo+IgM RF in the glomeruli. These results provide basic data for the establishment of Cryo GN although renal pathological changes are mild.

本研究旨在建立一种小鼠冷球蛋白血症性肾小球肾炎模型。我们建立了从Cryo GN患者中产生具有类风湿因子活性的IgM-κ crycoplulin（Cryo+IgM RF）的杂交瘤细胞系。我们向野生型Balb/c小鼠施用Cryo+IgM RF。尿白蛋白升高。肾组织病理学分析显示轻度毛细血管内增生、轻度系膜细胞增生和肾小球中Cryo+IgM RF沉积。这些结果为建立冷冻肾小球肾炎提供了基础资料，尽管肾脏病理变化轻微。

项目成果

SOMATIC HYPERMUTATION AND INTRACLONAL DIVERSITY IN MONOCLONAL IgM RHEUMATOID FACTOR IN MIXED CRYOGLOBUL INEMIA

混合性冷球性贫血中单克隆 IgM 类风湿因子的体细胞超突变和克隆内多样性

• 发表时间: 2009
• 作者: Kazunori Yamada;et al.
• 通讯作者: et al.

Somatic hypermutation and intraclonal diversity in monoclonal IgM rheumatoid factor in mixed cryoglobulinemia.

混合冷球蛋白血症中单克隆 IgM 类风湿因子的体细胞超突变和克隆内多样性。

• 发表时间: 2009
• 作者: Kazunori Yamada;Mitsuhiro Kawano;Ryo Inoue;Hiroshi Fujii;Kiyoaki Ito;Ryoko Hamano;Yasushi Kakuchi;Masami Matsumura;Ichiro Koni;Akihiro Yachie;Masakazu Yamagishi.
• 通讯作者: Masakazu Yamagishi.