Establishment of mouse model of human cryoglobulinemic glomerulonephritis

本研究旨在建立冷球蛋白血症(Cryo)肾小球肾炎(GN)的小鼠模型。我们建立了1例冷冻性肾炎患者产生具有类风湿因子活性的IgM-kappa冷球蛋白杂交瘤细胞系(Cryo IgM RF)。我们给野生型Balb/c小鼠注射了冷冻型IgM RF。尿白蛋白升高。肾组织病理分析显示轻度毛细血管内皮细胞增殖，系膜细胞轻度增殖，肾小球内有Cryo IgM RF沉积。这些结果为低温肾小球肾炎的建立提供了基础数据，尽管肾脏病变较轻。

The purpose of our study is to establish a mouse model of cryoglobulinemic (Cryo) glomerulonephritis (GN). We established the hybridoma cell line producing IgM-kappa cryoglobulin with rheumatoid factor activity (Cryo+IgM RF) from a patient with Cryo GN. We administered Cryo+IgM RF to wild type Balb/c mice. Urinary albumin increased. Pathological analysis of kidney tissue showed mild endocapillary proliferation, mild mesangial cell proliferation, and deposition of Cryo+IgM RF in the glomeruli. These results provide basic data for the establishment of Cryo GN although renal pathological changes are mild.