Establishment of mouse model of human cryoglobulinemic glomerulonephritis

本研究的目的是建立小鼠冷球蛋白血症（Cryo）型肾小球肾炎（GN）模型。我们从一例Cryo GN患者身上建立了产生具有类风湿因子活性的IgM-kappa冷球蛋白（Cryo+IgM RF）的杂交瘤细胞系。我们给野生型Balb/c小鼠注射Cryo+IgM RF。尿白蛋白升高。肾组织病理分析显示轻度毛细血管内增生，轻度系膜细胞增生，肾小球内可见Cryo+IgM RF沉积。虽然肾脏病理变化轻微，但这些结果为建立Cryo GN提供了基础资料。

The purpose of our study is to establish a mouse model of cryoglobulinemic (Cryo) glomerulonephritis (GN). We established the hybridoma cell line producing IgM-kappa cryoglobulin with rheumatoid factor activity (Cryo+IgM RF) from a patient with Cryo GN. We administered Cryo+IgM RF to wild type Balb/c mice. Urinary albumin increased. Pathological analysis of kidney tissue showed mild endocapillary proliferation, mild mesangial cell proliferation, and deposition of Cryo+IgM RF in the glomeruli. These results provide basic data for the establishment of Cryo GN although renal pathological changes are mild.