Establishment of mouse model of human cryoglobulinemic glomerulonephritis

本研究旨在建立一种小鼠冷球蛋白血症性肾小球肾炎模型。我们建立了从Cryo GN患者中产生具有类风湿因子活性的IgM-κ crycoplulin（Cryo+IgM RF）的杂交瘤细胞系。我们向野生型Balb/c小鼠施用Cryo+IgM RF。尿白蛋白升高。肾组织病理学分析显示轻度毛细血管内增生、轻度系膜细胞增生和肾小球中Cryo+IgM RF沉积。这些结果为建立冷冻肾小球肾炎提供了基础资料，尽管肾脏病理变化轻微。

The purpose of our study is to establish a mouse model of cryoglobulinemic (Cryo) glomerulonephritis (GN). We established the hybridoma cell line producing IgM-kappa cryoglobulin with rheumatoid factor activity (Cryo+IgM RF) from a patient with Cryo GN. We administered Cryo+IgM RF to wild type Balb/c mice. Urinary albumin increased. Pathological analysis of kidney tissue showed mild endocapillary proliferation, mild mesangial cell proliferation, and deposition of Cryo+IgM RF in the glomeruli. These results provide basic data for the establishment of Cryo GN although renal pathological changes are mild.