Anaerobic spores (C. perfringens) as a possible new fecal source tracking indicator of human fecal pollution.

产气荚膜梭菌比目前传统的粪便指示剂在各种水生环境中生存。因此，产气荚膜梭菌被认为是粪便污染的适宜指标。本研究利用cpe阳性产气荚膜梭菌的分布，描述了产气荚膜梭菌作为人类粪便污染源跟踪指标的新可能性。为评价产气荚膜芽孢杆菌溯源指示剂的效果，对污水和牲畜粪便中产气荚膜芽孢杆菌孢子的分布及其毒素类型进行了分析。采用多重PCR方法从牛、猪的污水、出水和粪便中分离出产气荚膜荚膜杆菌孢子。从生活污水和出水中检出cpe阳性产气荚膜梭菌的比例为30%。而家畜样品中只有1株牛分离株cpe基因阳性。cpe阳性产气荚膜梭菌的分布可作为人类粪便污染源的潜在追踪指标。

Clostridium perfringens survive in various aquatic environments than current traditional fecal indicator. Therefore, C. perfringens considered as suitable indicator for fecal pollution. This research described that the new possibility of C. perfringens as a useful source tracking indicator of human fecal pollution using distributions of cpe positive C. perfringens. To evaluate the efficacy of source tracking indicator of C. perfringens, distributions of C. perfringens spores and there toxin type in sewage and livestock fecal samples were analyzed. C. perfringens spores were isolated from sewage, effluents and fecal samples of cattle and pig by multiplex PCR. Almost all isolates were classified type A. From human sewage and effluents, cpe positive C. perfringens were detected at 30%. Whereas only 1 cattle isolate were positive cpe gene from livestock samples. Distributions of cpe positive C. perfringens were important as a potential source tracking indicator for human fecal pollution.