Eotaxin Immunoreactivity in the Nasal Polyps of Eosinophilic Chronic Rhinosinusitis Patients by using Laser capture microscope

在鼻息肉中，嗜酸性粒细胞活化趋化因子亚家族，嗜酸性粒细胞活化趋化因子-1，-2和-3的免疫反应性，注意到在大多数浸润性嗜酸性粒细胞，以及在其他炎症细胞，上皮细胞和内皮细胞。与非嗜酸性粒细胞CRS组相比，嗜酸性粒细胞CRS组的嗜酸性粒细胞中有显著的eotaxins表达。与非嗜酸性粒细胞CRS组相比，嗜酸性粒细胞CRS组中通过ELISA测量的鼻息肉和鼻窦积液的嗜酸性粒细胞趋化因子浓度显著增加。通过激光捕获显微切割，从富含嗜酸性粒细胞的上皮下组织中扩增单独的嗜酸性粒细胞活化趋化因子-3。

In the nasal polyps, immunoreactivities of the eotaxin subfamily, eotaxin-1, -2, and -3, were noted in most of the infiltrating eosinophils, as well as in other inflammatory cells, epithelial cells, and endothelial cells. Compared with non-eosinophilic CRS groups, eosinophilic CRS groups had a significant expression of eotaxins in their eosinophils. The eotaxin concentrations of nasal polyp and sinus effusion as measured by ELISA were significantly increased in the eosinophilic CRS group compared to the non-eosinophilic CRS group. By laser capture microdissection, eotaxin-3 alone was amplified from the eosinophil-rich subepithelial tissues.