Regulated maturation and controlled degradation: the life cycle of RNAs in Haloferax volcanii

在这个项目中，核糖核酸酶在嗜盐模式古菌Haloferax volcano ii将进行详细研究。RNA在细胞中作为前体分子产生，其必须经历一系列加工步骤以产生成熟的功能分子。此外，必须去除有缺陷或不再需要的RNA。核糖核酸酶是这些过程的负责者和必需品。在古细菌中，迄今为止只研究了少数核糖核酸酶，因此对这些过程知之甚少，本项目的研究将提供有关它们的新数据。实验将在H. volcanii，因为有多种分子生物学工具可用于该古菌，因此可以有效地进行研究。本计画中核糖核酸酶的分析将提供有关H. volcano ii，从而为古菌RNA代谢提供了重要的新数据。

In this project, ribonucleases in the halophilic model archaeon Haloferax volcanii will be studied in detail. RNAs are produced in the cell as precursor molecules that must undergo a series of processing steps to produce the mature functional molecule. In addition, RNAs that have defects or are no longer needed must be removed. Ribonucleases are responsible and essential for these processes. In archaea, only a few ribonucleases have been studied so far, so very little is known about these processes and the studies in this project will provide new data on them. The experiments will be carried out in H. volcanii, as a variety of molecular biology tools are available for this archaeon and thus the studies can be carried out efficiently. The analysis of ribonucleases in this project will provide information on RNA maturation and degradation in H. volcanii and thus provide important new data on RNA metabolism in archaea.