Investigation of the understudied CRISPR-Cas immune systems I-B and I-D in Haloarchaea
盐古菌中尚未研究的 CRISPR-Cas 免疫系统 I-B 和 I-D 的研究
基本信息
- 批准号:433108819
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2019
- 资助国家:德国
- 起止时间:2018-12-31 至 2023-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Although the CRISPR-Cas system has been identified as defence system of bacteria and archaea more than ten years ago, we still do not know all details about the molecular mechanisms of this immune system. There are currently 33 different variants of the CRISPR-Cas system known, all of them are defending the cell from invaders, but they do so with different sets of proteins and different molecular processes. While we know many details about a few variants, like the type I-E system from E. coli and the type II-A system from Streptococcus pyogenes, we now comparatively little about the other variants like type I-B and I-D. In addition, more is known about the bacterial systems than about the archaeal ones although only half of the bacteria contain a CRISPR-Cas system whereas almost all archaea encode one.Therefore we will investigate in this project the archaeal CRISPR-Cas systems I-B and I-D in Haloferax volcanii and Halorubrum lacusprofundi. Our preliminary results have revealed the details about the interference reaction of the Haloferax I-B system: the composition of the effector complex, PAM sequences and characteristics for a functional crRNA. In addition, we carried out the first experiments to characterise the I-D system in H. lacusprofundi and to study adaptation. Furthermore we could infect Halorubrum with viruses.In the frame of this project we want to analyse the I-D system of H. lacusprofundi in detail: the composition of its effector complex and the pre-requisites for an effective interference reaction (PAM sequences, characteristics for an active crRNA). H. lacusprofundi contains in addition to the I-D system also a I-B system and we want to investigate potential cross reactivities between both systems. Furthermore, we want to identify anti-CRISPR proteins for the I-B system. The adaptation step will also be investigated in Haloferax as well as in Halorubrum. For Haloferax we observed adaptation during self targeting and we will study this type of adaptation in detail. Using the viruses we found to infect Halorubrum we will study adaptation during viral infection in Halorubrum.Furthermore we will investigate the CRISPR associated CARF proteins. There are two CARF proteins in Haloferax, without known function and those we will study.We also want to develop a CRISPRa tool for activation of gene expression.
虽然CRISPR-Cas系统早在十多年前就被确定为细菌和古生菌的防御系统,但我们仍然不知道该免疫系统的所有分子机制。目前已知的CRISPR-Cas系统有33种不同的变体,所有这些变体都在保护细胞免受入侵者的侵袭,但它们通过不同的蛋白质组和不同的分子过程来实现这一点。虽然我们知道一些变异体的许多细节,比如大肠杆菌的I-E系统和化脓性链球菌的II-A系统,但我们现在对I-B和I-D等其他变异体的了解相对较少。此外,尽管只有一半的细菌含有CRISPR-Cas系统,而几乎所有的古生菌都编码CRISPR-Cas系统,但人们对细菌系统的了解比对古生菌系统的了解更多。因此,我们将在本项目中对Haloferax cancanii和Halorubrum lacus深部的古生菌CRISPR-Cas系统I-B和I-D进行研究。我们的初步结果揭示了Haloferax I-B系统的干扰反应的细节:效应复合体的组成、PAM序列和功能crRNA的特征。此外,我们还进行了第一次实验,以刻画拉氏沼虾的I-D系统,并研究其适应性。在这个项目的框架内,我们想要详细地分析梭状芽孢杆菌的I-D系统:其效应复合体的组成和有效干扰反应的先决条件(PAM序列,活性crRNA的特征)。除了I-D系统外,H.lacusprofundi还包含一个I-B系统,我们想要研究这两个系统之间的潜在交叉反应。此外,我们希望为I-B系统鉴定抗CRISPR蛋白。还将在Haloferax以及Halorubrum调查适应步骤。对于Haloferax,我们观察到了自定位期间的适应,我们将详细研究这种类型的适应。利用我们发现的感染卤虫的病毒,我们将研究病毒感染卤虫过程中的适应。此外,我们还将研究CRISPR相关的CARF蛋白。在Haloferax中有两个CARF蛋白,功能未知,我们将对其进行研究。我们还想开发一个CRISPRa工具来激活基因表达。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Professorin Dr. Anita Marchfelder其他文献
Professorin Dr. Anita Marchfelder的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Professorin Dr. Anita Marchfelder', 18)}}的其他基金
µ proteins in Haloferax volcanii: an important part of the proteome
Haloferax volcanii 中的 µ 蛋白质:蛋白质组的重要组成部分
- 批准号:
379070156 - 财政年份:2017
- 资助金额:
-- - 项目类别:
Priority Programmes
Regulated maturation and controlled degradation: the life cycle of RNAs in Haloferax volcanii
调控成熟和控制降解:Haloferax volcanii 中 RNA 的生命周期
- 批准号:
287106579 - 财政年份:2015
- 资助金额:
-- - 项目类别:
Research Grants
Small regulatory RNAs from the halophilic archaeon Haloferax volcanii
来自嗜盐古菌 Haloferax volcanii 的小调控 RNA
- 批准号:
238237704 - 财政年份:2013
- 资助金额:
-- - 项目类别:
Research Grants
Investigation of the prokaryotic immune systems I-B and I-D in archaea
古细菌原核免疫系统 I-B 和 I-D 的研究
- 批准号:
206942411 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Research Units
Unravelling the prokaryotic immune system
揭开原核免疫系统的神秘面纱
- 批准号:
206946519 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Research Units
RNA Structures as Processing Signals and Sensors
RNA 结构作为处理信号和传感器
- 批准号:
149178133 - 财政年份:2010
- 资助金额:
-- - 项目类别:
Priority Programmes
Identifizierung und Analyse wichtiger Teilschritte des RNA-Metabolismus in Archaeen und Pflanzen
古细菌和植物RNA代谢重要步骤的鉴定和分析
- 批准号:
66987035 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Heisenberg Professorships
Charakterisierung neuer Ribonukleasen in Haloferax volcanii
Haloferax volcanii 中新核糖核酸酶的表征
- 批准号:
61949542 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Research Grants
Small regulatory RNAs from the halophilic archaeon Haloferax volcanii
来自嗜盐古菌 Haloferax volcanii 的小调控 RNA
- 批准号:
39788743 - 财政年份:2007
- 资助金额:
-- - 项目类别:
Priority Programmes
Identifizierung und Analyse wichtiger Teilschritte des RNA-Metabolismus in Archaeen und Pflanzen
古细菌和植物RNA代谢重要步骤的鉴定和分析
- 批准号:
31970247 - 财政年份:2006
- 资助金额:
-- - 项目类别:
Heisenberg Fellowships
相似海外基金
Housing Experiences and Outcomes of an Understudied Urban Population
未充分研究的城市人口的住房经历和结果
- 批准号:
2328505 - 财政年份:2024
- 资助金额:
-- - 项目类别:
Standard Grant
Bivalent degraders of the understudied transcription factor TBXT for the rare cancer chordoma
正在研究的罕见癌症脊索瘤转录因子 TBXT 的二价降解剂
- 批准号:
10725821 - 财政年份:2023
- 资助金额:
-- - 项目类别:
United for Health Equity - Living PDX Program (U4HELPP)
United for Health Equity - Living PDX 计划 (U4HELPP)
- 批准号:
10733310 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Scalable platforms for understudied histone modifications and modifiers
用于未充分研究的组蛋白修饰和修饰剂的可扩展平台
- 批准号:
10567849 - 财政年份:2023
- 资助金额:
-- - 项目类别:
CE23-003 - Caregiver Relational Responsiveness (RR): An Understudied Fidelity Construct as a Mechanism to Increase Protective Factors Against Maltreatment.
CE23-003 - 照顾者关系响应 (RR):一种未经充分研究的忠诚度结构,作为增加针对虐待的保护因素的机制。
- 批准号:
10759625 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Statistical methods to understand changes in dietary patterns over time and cardiovascular disease risk among understudied populations
了解受研究人群饮食模式随时间变化和心血管疾病风险的统计方法
- 批准号:
10571108 - 财政年份:2023
- 资助金额:
-- - 项目类别:
High-throughput Discovery of Antibodies against Understudied Membrane Proteins
针对正在研究的膜蛋白的抗体的高通量发现
- 批准号:
10698472 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Leveraging the Electronic Health Record and Integrating Social and Biological Data to Expand Dementia Research in Understudied Populations in Los Angeles County
利用电子健康记录并整合社会和生物数据,扩大洛杉矶县未受研究人群的痴呆症研究
- 批准号:
10729950 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Identifying understudied protein-related glycoproteome disruption in Congenital Disorders of Glycosylation
识别先天性糖基化障碍中尚未研究的蛋白质相关糖蛋白组破坏
- 批准号:
10725869 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Alzheimer’s Disease and Related Dementias in The Most Incarcerated Generation: An Understudied Population with Health Disparities
被监禁最多的一代人中的阿尔茨海默氏病和相关痴呆症:健康差异尚未得到充分研究的人群
- 批准号:
10663035 - 财政年份:2023
- 资助金额:
-- - 项目类别: