Study on the molecular mechanisms of bone destruction by rheumatoid synovium by using comprehensive gene expression analysis and multiplex cytokine profiling .

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To clarify the molecular mechanism(s) of bone destruction by rheumatoid synovium, we analyzed the expression profile of gene and molecular markers including cytokines, growth factors, adhesion molecules, protein kinase, and transcription factors by comprehensive gene expression analysis and multiple analyte profiling in the culture of the synovial tissues derived from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). The higher number of osteoclasts was formed in the culture of rheumatoid synovium compared to the culture of OA synovium. In the culture of rheumatoid synovium, the increased gene expression of proinflammatory cytokines and IL-17F was observed and the pathway analysis showed the activation of NFκ-B and p38 Mark signaling. The increased production of TNFβand IL-17F by the cultured rheumatoid synovium was confirmed using multiplex cytokine profiling. The results indicate that the proinflammatory and T cell cytokine profiles differ in the culture of synovial tissues between RA and OA and this may contribute the excessive formation of osteoclasts in RA joints.