Inspection of receptor-Ig protein heterodimer for inhibition of its ligand

检查受体-Ig 蛋白异二聚体对其配体的抑制作用

• 批准号: 20591185
• 负责人: HANAWA Haruo
• 金额: $ 3.08万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591185/
• 关键词: 臨床免疫学; 生物学的製剤; 遺伝子治療; IL-1; 徙免疫疾患; IL-1受容体-Igヘテロダマー; アナキンラ; 鉄代謝; ヘム; 内因性リガンド; 自己免疫疾患; IL-1受容体-Igヘテロダイマー

Interleukin (IL)-1 inhibitor may be a candidate anti-inflammatory drug. We examined the effect of a heterodimer of IL-1 receptor accessory protein (Acp)-immunoglobulin (Ig) and IL-1R type II (IL1R2)-Ig named AcP-Ig/IL1R2-Ig heterodimer, and compared its effects with other IL-1 inhibitors reported previously. Our results demonstrated that the AcP-Ig/IL1R2-Ig heterodimer (rat, IC50=1.95 pM ; human, IC50=0.14 pM) inhibited IL-1 response to a greater extent than IL1RA (rat, IC50=1,935 pM), Acp-IL1R type I (IL1R1)-Ig homodimer (rat, IC50=73.7 pM ; human, IC50=4.48 pM) and Acp-IL1R2-Ig homodimer (rat IC50=72.8 pM) and strongly inhibited responses of both IL-1α and IL-1β.

白细胞介素-1抑制剂有可能成为一种候选的抗炎药物。我们研究了IL-1受体辅助蛋白（Acp）-免疫球蛋白（IG）和IL-1受体2（IL-1 R2）-IG的异源二聚体（AcP-IG/IL-1 R2-IG heterodimer）的作用，并将其作用与其他IL-1抑制剂进行了比较。我们的结果表明AcP-IG/IL 1 R2-IG异二聚体（大鼠，IC 50 =1.95 pM ;人，IC 50 =0.14 pM）抑制IL-1应答的程度大于IL 1 RA（大鼠，IC 50 = 1，935 pM），Acp-IL 1 R I型（IL 1 R1）-IG同型二聚体（大鼠，IC 50 =73.7 pM ;人，IC 50 =4.48 pM）和Acp-IL 1 R2-IG同源二聚体（大鼠IC 50 =72.8 pM），并且强烈抑制IL-1α和IL-1β两者的应答。

项目成果

Free heme is a danger signal血ducing expression of proinflammatory proteins in cultured cells derived from normal rat hearts

游离血红素是一种危险信号血液，在正常大鼠心脏的培养细胞中产生促炎蛋白的表达

• 期刊: Mol Immunol
• 影响因子: 3.6
• 作者: Hao K;Hanawa H;et al.
• 通讯作者: et al.

Autoimmune myocarditis and its mechanism

自身免疫性心肌炎及其机制

• 发表时间: 2010
• 作者: Haruo Hanawa;Tsuyoshi Yoshida;Rituo Wantanabe;Kazuhisa Hao;Limin Ding;Makoto Kodama;Yoshifusa Aizawa
• 通讯作者: Yoshifusa Aizawa

Free heme is a danger signal inducing expression of proinflammatory proteins in cultured cells derived from normal rat hearts.

• DOI: 10.1016/j.molimm.2011.02.013
• 发表时间: 2011-05
• 期刊: Molecular immunology
• 影响因子: 3.6
• 作者: Kazuhisa Hao;H. Hanawa;Limin Ding;Y. Ota;Kaori Yoshida;K. Toba;M. Ogura;Hiromi Ito;M. Kodama;Y. Aizawa

Free heme strongly induces expression of proinflammatory proteins in cultured cells derived from normal rat hearts

游离血红素强烈诱导正常大鼠心脏培养细胞中促炎蛋白的表达

• 发表时间: 2010
• 作者: Kazuhisa Hao;Haruo Hanawa;Limin Ding;ken Toba;Rituo Watanabe;Tsuyoshi Yoshida;Makoto Kodama;Yoshifusa Aizawa
• 通讯作者: Yoshifusa Aizawa

Lipocalin-2/Neutrophil Gelatinase-B Associated Lipocalin Is Strongly Induced in Hearts of Rats With Autoimmune Myocarditis and in Human Myocarditis

• DOI: 10.1253/circj.cj-09-0485
• 发表时间: 2010-03-01
• 期刊: CIRCULATION JOURNAL
• 影响因子: 3.3
• 作者: Ding, Limin;Hanawa, Haruo;Aizawa, Yoshifusa
• 通讯作者: Aizawa, Yoshifusa