Purified human plasma fibronectin prevents multi-drug resistant Pseudomonas aeruginosa infections.

纯化的人血浆纤连蛋白可预防多重耐药铜绿假单胞菌感染。

• 批准号: 20591559
• 负责人: KWON A-Hon
• 金额: $ 2.91万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591559/
• 关键词: ファイブロネクチン; 多剤耐性緑膿菌; 熱傷; 免疫低下; 糖尿病; 糖尿病マウス; 感染防御; 担癌マウス; 免疫低下マウス; 熱傷マウス

We investigated the protective action of plasma fibronectin (pFn) against burn wound sepsis caused by multi-drug resistant Pseudomonas aeruginosa (MDR P. aeruginosa). Mice with 30 % thermal injury, tumor bearing mice or immunosuppressive and streptozotocin-induced diabetic mice, were inoculated with MDR P. aeruginosa into, and immediately administered human pFn (FN group) or albumin (Alb group). In thermal injury model, 90 % of the mice in the Alb group had died, however, in the FN group survival was significantly improved to 80%. In immunosuppressive condition, all mice had died had diedin the Alb group, 60 % of the mice in the FN group was well survived. In diabetic model, survival rate in the Alb group and the FN group was 10% and 80%, respectively. At 6 and 24h after infection with the bacteria, the administration of pFn significantly depressed the count of viable bacteria within the liver and blood of mice compared with the Alb group. Supplementation with pFn increased the phagocytic activity against MDR P.aeruginosa in a dose-dependent manner. Single intravenous administration of pFn may serveas an effective strategy to prevent MDR P. aeruginosa infections in burn wounds,immunosuppressive condition and diabetic mellitus by enhancing the phagocytic activity of macrophages.

我们研究了血浆纤维连接蛋白（pFn）对多药耐药铜绿假单胞菌（MDR铜绿假单胞菌）引起的烧伤创面脓毒症的保护作用。将具有30%热损伤的小鼠、荷瘤小鼠或免疫抑制和链脲佐菌素诱导的糖尿病小鼠接种MDR铜绿假单胞菌，并立即施用人pFn（FN组）或白蛋白（Alb组）。在热损伤模型中，Alb组90%的小鼠死亡，而FN组的存活率显著提高至80%。在免疫抑制状态下，Alb组小鼠全部死亡，FN组小鼠存活率为60%。在糖尿病模型中，Alb组和FN组的存活率分别为10%和80%。在细菌感染后6和24小时，与Alb组相比，给予pFn显著降低了小鼠肝脏和血液中的活菌计数。补充pFn以剂量依赖性方式增加细胞对MDR铜绿假单胞菌的吞噬活性。pFn单次静脉给药可能通过增强巨噬细胞的吞噬活性，预防烧伤创面、免疫抑制状态和糖尿病患者的MDR铜绿假单胞菌感染。

项目成果

Protective effect of neutrophil elastase inhibitor (FR136706) in lethal acute liver failure induced by D-galactosamine and lipopolysaccharide in rats.

中性粒细胞弹性蛋白酶抑制剂（FR136706）对 D-半乳糖胺和脂多糖诱导的大鼠致死性急性肝衰竭的保护作用。

• 期刊: J Surg Res (in press)
• 作者: Uchida Y;Kaibori M
• 通讯作者: Kaibori M

Effects of medicinal mushroom (Sparassis crispa) on the wound healing in streptozotocin-induced diabetic rats.

药用蘑菇（Sparassis Cripa）对链脲佐菌素诱导的糖尿病大鼠伤口愈合的影响。

• 发表时间: 2009
• 期刊: American Journal of Surgery 197(4)
• 作者: Kwon AH;Qiu Z;Kimura T;Yamamoto K;Hashimoto M
• 通讯作者: Hashimoto M

Edarvone prevents Fas-induced fulminant hepatic failure in mice by regulating mitochondrial Bcl-xL and Bax.

Edarvone 通过调节线粒体 Bcl-xL 和 Bax 来预防 Fas 诱导的暴发性肝衰竭。

• 发表时间: 2008
• 期刊: Shock 30(2)
• 作者: Miyaso T;Kwon AH;Tsuji K;Qiu Z;Okumura T;Kamiyama Y
• 通讯作者: Kamiyama Y

Involvement of the functional food Oligonol in hepatoprotection by reduction of nitric oxide.

功能性食品 Oligonol 通过减少一氧化氮参与肝脏保护。

• 发表时间: 2009
• 作者: Nishizawa M;Yamanishi R;Miura T;權雅憲;Okumura T
• 通讯作者: Okumura T

Adult bone marrow cells can differentiate into hemopoietic cells and endothelial cells but not into other lineage cells in normal growth and normal life.

成体骨髓细胞在正常生长和生活中可以分化为造血细胞和内皮细胞，但不能分化为其他谱系细胞。

• 发表时间: 2010
• 期刊: International Journal of Hematology 91(2)
• 作者: Yanai S;Adachi Y;Shi M;Shigematsu A;Shima C;Imai Y;Kwon AH;Ikehara S
• 通讯作者: Ikehara S