The effects and possible mechanism of syngeneic bone marrow mononuclear cell transplantation on pulmonary arterial hypertension

同基因骨髓单个核细胞移植对肺动脉高压的影响及可能机制

• 批准号: 20591649
• 负责人: KITAICHI Takashi
• 金额: $ 2.25万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591649/
• 关键词: 骨髄単核球細胞移植; 肺高血圧治療; 肺動脈再構築; VEGF-VEGF receptor; 肺小動脈リモデリング; 再生医療; 細胞治療; 血管新生; 肺高血圧; 循環器

We investigated the effects and possible mechanism of syngeneic bone marrow mononuclear cell (BM-MNC) transplantation on pulmonary arterial hypertension induced by monocrotaline. Monocrotaline-injected mice improved significantly after BM-MNC transplantation compared with those of mice after monocrotaline injection. However, BM-MNCs were not incorporated into the lung at1week after transplantation, and significant vascular endothelial growth factor (VEGF) upregulation and without receptor expression was observed in lung tissue after transplantation. Improvement of pulmonary arterial hypertension was inhibited by simultaneous administration of VEGF receptor-2inhibitor with BM-MNC transplantation. These results indicate that syngeneic BMMNC transplantation improves monocrotaline-induced pulmonary arterial hypertension by favorable pulmonary artery remodeling through VEGF upregulation.

目的：探讨同基因骨髓单个核细胞（BM-MNC）移植对野百合碱诱导的肺动脉高压的影响及其可能机制。与注射野百合碱的小鼠相比，注射野百合碱的小鼠在BM-MNC移植后明显改善。但移植后1周BM-MNCs未进入肺组织，移植后肺组织中血管内皮生长因子（VEGF）表达明显上调，且无受体表达。BM-MNC移植同时应用VEGF受体-2抑制剂可抑制肺动脉高压的改善。这些结果表明，同基因BMMNC移植改善野百合碱诱导的肺动脉高压，通过VEGF上调有利的肺动脉重构。

项目成果

経静脈的な同種骨髄単核球細胞移植のmonocrotaline誘発肺高血圧に対する肺血管床改善効果

静脉同种异体骨髓单个核细胞移植改善肺血管床对野百合碱性肺动脉高压的影响

• 发表时间: 2008
• 作者: Yoshida H;Kitaichi T;Urata M;Kurobe H;Kanbara T;Motoki T;Kitagawa T;吉田誉;吉田誉;吉田誉;Homare Yoshida;吉田誉;吉田 誉;吉田誉;吉田 誉;吉田誉
• 通讯作者: 吉田誉

Intravenous transplantation of syngenic bone marrow mononuclear cells improves monocrotaline induced pulmonary artery hypertension in mice : therapeutic angiogenesis through VEGF-VEGF receptor system

同源骨髓单核细胞静脉移植可改善野百合碱诱导的小鼠肺动脉高压：通过 VEGF-VEGF 受体系统治疗性血管生成

• 发表时间: 2009
• 作者: Yoshida H;Kitaichi T;Urata M;Kurobe H;Kanbara T;Motoki T;Kitagawa T;吉田誉;吉田誉;吉田誉;Homare Yoshida
• 通讯作者: Homare Yoshida

Syngeneic bone marrow mononuclear cells improve pulmonary arterial hypertension through vascular endothelial growth factor upregulation

同基因骨髓单核细胞通过上调血管内皮生长因子改善肺动脉高压

• 发表时间: 2009
• 期刊: Annals of Thoracic Surgery
• 影响因子: 4.6
• 作者: Yoshida H;Kitaichi T;Urata M;Kurobe H;Kanbara T;Motoki T;Kitagawa T
• 通讯作者: Kitagawa T

経静脈的骨髄単核球細胞移植はVEGF-VEGFR 系を介する血管新生により肺高血圧症を改善する

静脉骨髓单核细胞移植通过 VEGF-VEGFR 系统的血管生成改善肺动脉高压

• 发表时间: 2008
• 作者: Yoshida H;Kitaichi T;Urata M;Kurobe H;Kanbara T;Motoki T;Kitagawa T;吉田誉;吉田誉;吉田誉;Homare Yoshida;吉田誉;吉田 誉
• 通讯作者: 吉田 誉

同種骨髄単核球細胞移植はVEGFの発現増強を介して肺高血圧症を改善する

同种异体骨髓单核细胞移植通过增强 VEGF 表达改善肺动脉高压

• 发表时间: 2009
• 作者: Yoshida H;Kitaichi T;Urata M;Kurobe H;Kanbara T;Motoki T;Kitagawa T;吉田誉
• 通讯作者: 吉田誉