The effects and possible mechanism of syngeneic bone marrow mononuclear cell transplantation on pulmonary arterial hypertension

探讨同基因骨髓单个核细胞(BM-MNC)移植对野百合碱诱导的大鼠肺动脉高压的影响及其可能机制。注射野百合碱的小鼠在BM-MNC移植后较注射野百合碱的小鼠显著改善。然而，移植后1周，BM-MNCs仍未进入肺内，移植后肺组织中血管内皮生长因子(VEGF)表达明显上调，且无受体表达。联合应用血管内皮生长因子受体-2抑制剂和BM-MNC移植可抑制大鼠肺动脉高压的改善。这些结果表明，同基因BMMNC移植通过上调血管内皮生长因子，改善野百合碱所致的肺动脉高压。

We investigated the effects and possible mechanism of syngeneic bone marrow mononuclear cell (BM-MNC) transplantation on pulmonary arterial hypertension induced by monocrotaline. Monocrotaline-injected mice improved significantly after BM-MNC transplantation compared with those of mice after monocrotaline injection. However, BM-MNCs were not incorporated into the lung at1week after transplantation, and significant vascular endothelial growth factor (VEGF) upregulation and without receptor expression was observed in lung tissue after transplantation. Improvement of pulmonary arterial hypertension was inhibited by simultaneous administration of VEGF receptor-2inhibitor with BM-MNC transplantation. These results indicate that syngeneic BMMNC transplantation improves monocrotaline-induced pulmonary arterial hypertension by favorable pulmonary artery remodeling through VEGF upregulation.