ROLE OF CALPAIN IN PATIENTS WITH RECURRENT MISCARRIAGE

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We investigated the existence and contribution of anti-calpastatin antibody and correlation with antiphoshatidylethanolaine (anti-PE) antibody in serum of recurrent miscarriage. Subject group was patients with history of recurrent miscarriage and control group was healthy women with informed consent. We performed immunohistochemistry using mid-phase endometrium to investigate localization of calpain and calpastatin. ELISA was performed to measure anti-calpastatin antibody and PE-binding protein dependent IgG antibody in sera of non-pregnancy period in each group. Staining of μ-calpain and calpastatin were observed in the cytoplasm of stromal and epithelial cells in mid-phase endometrium but no significant differences were observed in staining patterns. Subject group of anti-calpastatin antibody in sera was 4.317±0.987 (U/ml) and control group was 3.373±0.653. Subject group was significantly higher than control group. There was no significant correlation between anti-calpastatin antibody and anti-PE antibody. The result provided that calpain-calpastatin system may plays important role in endometrium of recurrent miscarriage independently from anti-PE antibody-calpain pathway.