Anti-aging research on the oocyte reproductive function based on the gene expression profiling

线粒体以细胞器的形式存在于细胞中，参与氧化磷酸化作用，为细胞提供三磷酸腺苷。我们的研究表明，来自40岁以上女性的未受精卵母细胞和未卵裂胚胎的mtDNA拷贝数明显较低。线粒体DNA拷贝数与卵裂球细胞体积呈正相关。从胚胎形态分类与胚胎发育之间的关系出发，对斑马鱼胚胎质量进行了研究。随着年龄的增长，劣型胚胎发光延迟，胚胎发育效率有降低的趋势。从卵母细胞凋亡的角度，应用基因芯片技术对斑马鱼caspase基因的表达进行了研究。半胱氨酸天冬氨酸氨基转移酶基因表达增加，提示胚胎发生了细胞凋亡。

Mitochondria exists in cell as an organelle that takes part in the role of oxidative phosphorylation to supply ATP to the cell. Our studies demonstrated that lower mtDNA copy number existed in unfertilized oocytes and uncleavaged embryos originated from over 40 years of age women significantly. A positive correlation was demonstrated between mtDNA copy number and the cell volume of blastomere. We examined the quality of embryo in zebrafish, in terms of the implication between morphological classification of embryo and embryonic development. Glowing of poor form embryo was delayed and the efficiency of embryonic development tended to be reduced by aging. From the respect of apoptosis on the oocyte, microarray analysis was applied to caspase gene expression in zebrafish. Embryo was revealed in apoptosis through the increased expression of caspase genes.