Are odontogenesis-related genes detected with microarray really involved in odontogenesis?

微阵列检测到的牙发育相关基因真的参与牙发育吗？

• 批准号: 20592151
• 负责人: MURAMATSU Takashi
• 金额: $ 2.58万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592151/
• 关键词: 歯牙形成性能; 歯胚; 歯乳頭; 遺伝子導入; 歯牙再生; 歯牙形成; Adamts4; Aldh1a2; Lef-1; 遺伝子解析; in situ hybridization; Aldhla2

Important factors involved in odontogenesis in mouse dental papillae disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that certain genes involved in odontogenesis in dental papillae were subject to pre-/post-natal down-regulation. Our goal was to identify, by microarray analysis, which genes were down-regulated. Dental papillae were isolated from embryonic 16-day-, 18-day- (E16, E18), and post-natal 3-day-old (P3) murine first mandibular molar germs and analyzed by microarray. The number of down-regulated genes was 2269 between E16 and E18, and 3130 between E18 and P3. Drastic down-regulation (fold change > 10.0) of Adamts4, Aldha1a2, and Lef1 was observed at both E16 and E18, and quantitative RT-PCR revealed a post-natal reduction in their expression (Adamts4, 1/3; Aldh1a2, 1/13; and Lef1, 1/37). These results suggest that down-regulation of these three genes is an important factor in normal odontogenesis in dental papillae. To confirm whether these genes are involved in odontogenesis or not, we constructed expression vectors of these genes. We transfected the genes into adult pulp cells and combined with dental epithelium, and transplanted under kidney capsule. However, odontogenesis could not be seen histologically. Further investigation is necessary to confirm that these genes are involved in odontogenesis.

小鼠牙乳头发育过程中的重要因素在出生前和出生后的发育阶段之间消失。因此，我们假设牙齿乳头中涉及牙齿形成的某些基因在出生前/出生后受到下调。我们的目标是通过微阵列分析确定哪些基因下调。从胚胎16天、18天(E16、E18)和出生后3天(P3)的小鼠下颌第一磨牙胚胎中分离出牙乳头，并进行基因芯片分析。E16~E18间下调基因数为2269个，E18~P3间为3130个。在E16和E18观察到Adamts4、Aldha1a2和Lef1的急剧下调(折叠变化和10.0)，定量RT-PCR显示它们的表达在出生后减少(Adamts4，1/3；Aldh1a2，1/13；Lef1，1/37)。这些结果表明，这三个基因的下调是牙齿乳头正常发育的一个重要因素。为了确定这些基因是否参与牙齿发育，我们构建了这些基因的表达载体。将基因导入成人牙髓细胞，与牙体上皮结合，移植于肾被膜下。然而，在组织学上看不到牙齿发育。需要进一步的研究来确认这些基因与牙齿发育有关。

项目成果

Molecular interactions and cellular events involved in palatal rugae development

腭皱发育中涉及的分子相互作用和细胞事件

• 发表时间: 2009
• 作者: Sohn WJ;Jung HI;Choi MA;Yamamoto H;Shin HI;Lee SG;Jung HS;Kim JY
• 通讯作者: Kim JY

唾液を用いた口腔癌のスクリーニング検査-プロテオームによるenolase-1の発現の解析-

使用唾液进行口腔癌筛查试验 - 使用蛋白质组分析 enolase-1 表达 -

• 发表时间: 2008
• 作者: 片倉朗;作間巧;菅原圭亮;恩田健志;高木亮;神山勲;大野啓介;高野伸夫;柴原孝彦;村松敬
• 通讯作者: 村松敬

Periodontal tissue formation by reaggregation system in mice

小鼠重聚集系统形成牙周组织

• 发表时间: 2009
• 期刊: J Hard Tissue Biology 18(2)
• 作者: Yamamoto H;Cai J;Cho SW;Kim JY;Jung HS
• 通讯作者: Jung HS

横口蓋ヒダの有無による口蓋粘膜構造の違いについて

腭粘膜结构的差异取决于是否存在侧腭皱襞

• 发表时间: 2009
• 作者: 山本仁;新美寿英;太田ルミ;鈴木久仁博;寒河江登志朗;小澤幸重
• 通讯作者: 小澤幸重

象牙芽細胞のTRPV1チャネルを介したCa^<2+>シグナル

成牙本质细胞中通过 TRPV1 通道的 Ca^<2+> 信号

• 发表时间: 2009
• 作者: 津村麻記;澁川義幸;市川秀樹;村松敬;下野正基;田崎雅和
• 通讯作者: 田崎雅和