Establishment of invasion/metastasis inhibition therapy against oral squamous cell carcinomas : a basic study.

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Multicellular aggregates of MOK205, an oral squamous carcinoma cell line, transformed to monolayer colonies on laminin 5 and type 1 collagen substrates, and then showed cell dispersion only on laminin 5. This phenomenon was triggered by the increased motility and disruption of E-cadherin-mediated intercellular adhesion via the activation of integrin receptor by laminin 5. When PP1, a specific inhibitor of the Src family, was added to the cell scattering model of MOK205, cell dispersion was almost completely blocked, suggesting that Src was involved in the interaction between integrin and cadherin. In addition, fluorescence microscopic observation revealed that phospho-tyrosine 215-Src and phospho-tyrosine 529-Src were localized along the cell membrane of the intercellular adhesion of MOK205 immediately before cells started dispersion on laminin 5. Taken together, these results suggested that Src could be a target of the invasion/metastasis inhibition therapy against oral squamous cell carcinomas.