Characterization of putative amelogenic cells and mesenchymal cells isolated from human dental follicle and dental papilla

从人牙囊和牙乳头中分离的推定成釉细胞和间充质细胞的表征

• 批准号: 20592267
• 负责人: KANYAMA Manabu
• 金额: $ 2.83万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592267/
• 关键词: 上皮間葉相互作用; 象牙質再生; 人工歯根; 象牙質; 歯根再生

In this study, we isolated human dental follicle epithelial (DFE) cells that were different from gingival epithelial (GE) cells in terms of their gene expression profiles and proliferative capacity. Furthermore, DFE cells were revealed to be putative amelogenic cells based on their gene expression of an ameloblast marker, amelogenin. We found that a direct contact between DFE and dental papilla mesenchymal (DPM) cells increased an amelogenin expression in DFE cells, suggesting transmembrane signaling from the DPM cells played some pivotal roles for DFE cells to enter into the ameloblast lineage. More importantly, this increase was remarkable when DFE cells were cultured with DPM cells in comparison to DFM cells. We confirmed that DPM cells expressed dentin sialophosphoprotein (DSPP), one of odontoblast markers, but DFM cells did not. Therefore, this DPM cells-driven DFE cells differentiation might be a reproduction of epithelial - mesenchymal interaction, found in tooth generation. However, the underlying mechanisms of this induced differentiation of DFE cells were not cleared. Much more signaling works are required to elucidate epithelial - mesenchymal interaction in human tooth generation. Novel human amelogenic cells, DFE cells, might be useful tool for such kind of signaling works.

在这项研究中，我们分离了人牙囊上皮（DFE）细胞，其基因表达谱和增殖能力与牙龈上皮（GE）细胞不同。此外，根据成釉细胞标记物釉原蛋白的基因表达，DFE 细胞被认为是推定的釉原细胞。我们发现，DFE 和牙乳头间充质 (DPM) 细胞之间的直接接触增加了 DFE 细胞中牙釉原的表达，表明来自 DPM 细胞的跨膜信号传导对于 DFE 细胞进入成釉细胞谱系发挥了一些关键作用。更重要的是，与 DFM 细胞相比，当 DFE 细胞与 DPM 细胞一起培养时，这种增加是显着的。我们证实 DPM 细胞表达牙本质唾液酸磷蛋白 (DSPP)，这是成牙本质细胞标记物之一，但 DFM 细胞不表达。因此，这种 DPM 细胞驱动的 DFE 细胞分化可能是牙齿生成中发现的上皮-间质相互作用的再现。然而，这种诱导 DFE 细胞分化的潜在机制尚不清楚。需要更多的信号工作来阐明人类牙齿生成中的上皮-间质相互作用。新型人类致釉细胞 DFE 细胞可能是此类信号传导工作的有用工具。

项目成果

Functional Regeneration of Oral Tissue-Transfer of in vitro Results with Cultured Stem/Progenitor Cells to in vivo-.

口腔组织的功能再生-将培养的干细胞/祖细胞的体外结果转移到体内-。

• 发表时间: 2010
• 作者: 谷口誠;有本隆文;五十嵐武;窪木拓男;園山亘;園山亘
• 通讯作者: 園山亘

Sonic Hedgehog Positively Regulates Odontoblast Differentiation by a BMP2/4-dependent Mechanism.

Sonic Hedgehog 通过 BMP2/4 依赖性机制积极调节成牙本质细胞分化。

• 发表时间: 2009
• 期刊: Journal of Oral Tissue Engineering 7(1)
• 作者: Shimo T;Koyama E;Kanyama M;Kurio N;Okui T;Yamamoto D;Hassan NMM;Sasaki A
• 通讯作者: Sasaki A

発生期歯胚において発現する新規遺伝子群の同定とその発現パターン解析

发育中牙胚中表达的新基因组的鉴定及其表达模式分析

• 发表时间: 2009
• 期刊: 岡山歯学会雑誌 28(印刷中)
• 作者: Y. Okamoto;W. Sonoyama;M. Ono;K. Akiyama;T. Fujisawa;M. Oshima;Y. Tsuchimoto;Y. Matsuka;T. Yasuda;S. Shi;T. Kuboki;内部健太
• 通讯作者: 内部健太

Characterization of putative amelo- genic cells isolated from human dental follicle

从人牙囊中分离出的推定釉原细胞的表征

• 发表时间: 2008
• 作者: Y. Tsuchimoto;W. Sonoyama;Y. Okamoto;M. Oshima;Y. Matsuka;T. Kuboki
• 通讯作者: T. Kuboki