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Construction of a novel taste sensor using the taste receptor protein of vertebrate origin

Construction of a novel taste sensor using the taste receptor protein of vertebrate origin
利用脊椎动物来源的味觉受体蛋白构建新型味觉传感器
批准号:
20688015
负责人:
MISAKA Takumi
金额:
$16.31万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (A)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2009

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中文摘要
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英文摘要
We planned to construct a novel taste sensor using the taste receptor protein. The taste censor should be present a stable score to the tastants. To measure the sweetness, we generated a cell line that were stably expressing the human sweet taste receptor (hT1R2/hT1R3). The cell could be worked as a sensor for sweet taste to obtain the change of fluorescent intensities of the intracellular calcium indicatordye.
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会议论文
Amiloride reduces the sweet taste intensity by inhibiting the human sweet taste receptor Biochem.
阿米洛利通过抑制人类甜味受体 Biochem 来降低甜味强度。
DOI: --
发表时间: 2010
期刊: Biophys. Res. Commun. (in press)
影响因子: --
作者: [Imada, T., Misaka, T., Fujiwara, S., Okada, S., Fukuda, Y., Abe, K.]
通讯作者: K.
DOI: 10.1016/j.bbrc.2009.05.069
发表时间: 2009-07-31
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子: 3.1
作者: [Ishii, Sho, Misaka, Takumi, Abe, Keiko]
通讯作者: Abe, Keiko
甘味受容体発現コンストラクト、これを発現させた細胞体、及びその利用
甜味受体表达构建体、表达其的细胞体及其用途
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: []
通讯作者:
pH-Dependent structural change in neoculin with special reference to its taste-modifying activity
Neoculin 的 pH 依赖性结构变化,特别是其味道调节活性
DOI: --
发表时间: 2009
期刊: Biosci.Biotechnol.Biochem 73
影响因子: --
作者: [Morita Y, Nakajima K, Iizuka K, Terada T, Shimizu-Ibuka A, Ito K, Koizumi A, Asakura T, Misaka T, Abe K]
通讯作者: Abe K
8
    Characterization of chemical compounds contributing to the taste enhancer/suppressor using the taste receptor-expressing cells and examination of those molecular mechanisms
    • 批准号:
      16H04918
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $11.23万
    • 财政年份:
      2016
    • 负责人:
      MISAKA Takumi
    • 依托单位:
    Construction of a novel fluorescent measurement system to evaluate pH values in fluorescent imaging analysis
    • 批准号:
      15K14914
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.5万
    • 财政年份:
      2015
    • 负责人:
      MISAKA Takumi
    • 依托单位:
    海外基金