Isolation of HSC progenitors in in vitro ES cell differentiation culture system.

我们建立了RVG 25 ES细胞系，可以同时监测Runx 1和Gata 1的表达。在ES细胞造血分化培养中，我们发现Runx 1 + Gata 1 +VE-cadherin+细胞具有高的产生定型造血细胞（B细胞）的能力。此外，我们发现E7.5小鼠卵黄囊中的VE-钙粘蛋白+细胞可以分化为B细胞。此外，我们制作了Gata 1-IRES-MerCreMer小鼠品系。使用小鼠，我们可以随时标记Gata 1表达细胞。

We established RVG25 ES cell lines which can monitor expression of Runx1 and Gata1 at the same time. In the ES cell hematopoietic differentiation culture, we found that Runx1+Gata1+VE-cadherin+ cells had high ability to produce definitive hematopoietic cells (B cells). Additionally, we showed that VE-cadherin+ cells in E7.5 murine yolk sac could differentiate into B cells. Furthermore we made Gata1-IRES-MerCreMer mouse lines. Using the mice, we can label Gata1-expressing cells at any time.