Isolation of HSC progenitors in in vitro ES cell differentiation culture system.

建立了可同时监测RUNX1和GATA1表达的RVG25 ES细胞系。在ES细胞的造血祖细胞分化培养中，我们发现RUNX1 GATA1 VE-cadherin细胞具有很高的定向造血细胞(B细胞)的生成能力。此外，我们还发现E7.5小鼠卵黄囊中的VE-cadherin细胞可以分化为B细胞。此外，我们还建立了GATA1-IRES-MerCreMer小鼠品系。利用小鼠，我们可以随时标记表达GATA1的细胞。

We established RVG25 ES cell lines which can monitor expression of Runx1 and Gata1 at the same time. In the ES cell hematopoietic differentiation culture, we found that Runx1+Gata1+VE-cadherin+ cells had high ability to produce definitive hematopoietic cells (B cells). Additionally, we showed that VE-cadherin+ cells in E7.5 murine yolk sac could differentiate into B cells. Furthermore we made Gata1-IRES-MerCreMer mouse lines. Using the mice, we can label Gata1-expressing cells at any time.