Isolation of HSC progenitors in in vitro ES cell differentiation culture system.

在体外 ES 细胞分化培养系统中分离 HSC 祖细胞。

• 批准号: 20890285
• 负责人: TANAKA Yosuke
• 金额: $ 2.11万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20890285/
• 关键词: Runx1; Gata1; VE-cadherin; GATA1; ES細胞

We established RVG25 ES cell lines which can monitor expression of Runx1 and Gata1 at the same time. In the ES cell hematopoietic differentiation culture, we found that Runx1+Gata1+VE-cadherin+ cells had high ability to produce definitive hematopoietic cells (B cells). Additionally, we showed that VE-cadherin+ cells in E7.5 murine yolk sac could differentiate into B cells. Furthermore we made Gata1-IRES-MerCreMer mouse lines. Using the mice, we can label Gata1-expressing cells at any time.

我们建立了能同时监测Runx 1和Gata 1表达的RVG 25 ES细胞系。在ES细胞造血分化培养中，我们发现Runx 1 + Gata 1 +VE-cadherin+细胞具有高的产生定型造血细胞（B细胞）的能力。此外，我们发现E7.5小鼠卵黄囊中的VE-钙粘蛋白+细胞可以分化为B细胞。此外，我们制作了Gata 1-IRES-MerCreMer小鼠品系。使用小鼠，我们可以随时标记Gata 1表达细胞。

项目成果

Investigation of lymphoid potential in early VE-cadherin positive population

早期VE-钙粘蛋白阳性人群淋巴潜能的调查

• 发表时间: 2010
• 作者: 林真由美;中村昭代鹿野佐代子;田中洋介
• 通讯作者: 田中洋介