The health effects of dietary glycated protein by ascorbic acid

抗坏血酸膳食糖化蛋白的健康影响

• 批准号: 21500797
• 负责人: MIYAKE Noriko
• 金额: $ 2.83万
• 依托单位: Tokyo Kasei Gakuin University (2011)Nigata University of Phermacy and Applied Life Sciences (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500797/
• 关键词: ビタミンC; 酸化; メイラード反応; 加熱調理; AGE受容体; 安全性; 培養細胞; 細胞増殖; α-カルボニル化合物; 食品

The health effects of food-derived glycated casein by ascorbic acid(AsA) oxidation products were in vitro investigated. AsA, 2, 3-diketogulonic acid(DKG), glucose, glyoxal(GO) and methylglyoxal(MGO) were heated with casein, and their yields of glycated products were determined. Formation of DKG-casein was at similar level to GO-and MGO-casein. The cytotoxicity of DKG-casein was examined using intestinal cell line Caco-2.The larger inhibitory effect in Caco-2 cellular proliferation by DKG-casein was observed than GO-and MGO-casein. The expression of the receptor for advanced glycation endproducts(RAGE) mRNA in Caco-2 was studied. Stimulation of Caco-2 with DKG-casein resulted in a significant increase in RAGE mRNA expression. Therefore, dietary ascorbic acid-derived glycation products might have a risk for human health and the effect was suggested to be mediated by RAGE.

体外研究了抗坏血酸（AsA）氧化产物对食源性糖化酪蛋白的健康影响。用酪蛋白加热AsA、2,3 -二酮gulonic acid（DKG）、葡萄糖、乙二醛（GO）和甲基乙二醛（MGO），测定其糖基化产物的产率。dkg -酪蛋白的形成水平与go和mgo -酪蛋白相似。采用肠细胞系Caco-2检测dkg -酪蛋白的细胞毒性。dkg -酪蛋白对Caco-2细胞增殖的抑制作用大于go -和mgo -酪蛋白。研究了晚期糖基化终产物受体（RAGE） mRNA在Caco-2中的表达。dkg -酪蛋白刺激Caco-2导致RAGE mRNA表达显著增加。因此，饮食中抗坏血酸衍生的糖基化产品可能对人体健康有风险，并可能由RAGE介导。

项目成果

食品の調理・加工過程におけるビタミンC由来グリケーション反応生成物の健康への影響

食品烹饪和加工过程中维生素 C 衍生的糖化反应产物对健康的影响

• 发表时间: 2011
• 作者: 三宅紀子;酒井清子;松井裕巳;吉川巧;鈴木恵美子;倉田忠男
• 通讯作者: 倉田忠男

アスコルビン酸酸化生成物の関与するタンパク質のグリケーション反応

涉及抗坏血酸氧化产物的蛋白质糖化反应

• 发表时间: 2010
• 作者: 三宅紀子;他;三宅紀子
• 通讯作者: 三宅紀子