The health effects of dietary glycated protein by ascorbic acid

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The health effects of food-derived glycated casein by ascorbic acid(AsA) oxidation products were in vitro investigated. AsA, 2, 3-diketogulonic acid(DKG), glucose, glyoxal(GO) and methylglyoxal(MGO) were heated with casein, and their yields of glycated products were determined. Formation of DKG-casein was at similar level to GO-and MGO-casein. The cytotoxicity of DKG-casein was examined using intestinal cell line Caco-2.The larger inhibitory effect in Caco-2 cellular proliferation by DKG-casein was observed than GO-and MGO-casein. The expression of the receptor for advanced glycation endproducts(RAGE) mRNA in Caco-2 was studied. Stimulation of Caco-2 with DKG-casein resulted in a significant increase in RAGE mRNA expression. Therefore, dietary ascorbic acid-derived glycation products might have a risk for human health and the effect was suggested to be mediated by RAGE.